中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (3): 487-492.doi: 10.4103/1673-5374.179068

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

丹酚酸B如何促进脊髓损伤后轴突髓鞘的再生?

  

  • 收稿日期:2016-02-20 出版日期:2016-03-15 发布日期:2016-03-15
  • 基金资助:

    广东医学院基金(XB1380)

Salvianolic acid B protects the myelin sheath around injured spinal cord axons

Zhe Zhu 1, Lu Ding 2, Wen-feng Qiu 2, Hong-fu Wu 2, Rui Li 1   

  1. 1 Hand & Foot Surgery and Reparative & Reconstruction Surgery Center, the Second Hospital of Jilin University, Changchun, Jilin Province, China
    2 Department of Physiology, Guangdong Medical University, Dongguan, Guangdong Province, China
  • Received:2016-02-20 Online:2016-03-15 Published:2016-03-15
  • Contact: Rui Li, M.D. or Hong-fu Wu, Ph.D., 13304321102@qq.com or hongfuw@126.com.
  • Supported by:

    This study was supported by a grant of Guangdong Medical University of China, No. XB1380.

摘要:

丹酚酸B作为药物丹参的有效成分,在脑损伤及脊髓损伤动物模型中证实有神经保护作用,可促进神经功能的恢复,但其对脊髓损伤后髓鞘保护作用尚不明确。为此,实验首先进行了体外实验,证实丹酚酸B有促进少突胶质前体细胞分化的作用,且以剂量为20mg/kg时作用最为明显。其后,在体内实验给予脊髓损伤大鼠腹腔注射20mg/kg丹酚酸B干预8周发现脊髓组织中轴突髓鞘和再生轴突的数量增加,且大鼠神经功能恢复,同时可使损伤脊髓中细胞凋亡执行者caspase-3表达明显下降。由此,我们认为丹酚酸B对损伤脊髓的神经轴突髓鞘有保护作用,可促进神经功能的恢复,其作用途径可能与抑制细胞凋亡、促进少突胶质前体细胞的成熟分化有关。

关键词: 神经再生, 脊髓损伤, 丹酚酸B, 少突胶质前体细胞, 髓鞘

Abstract:

Salvianolic acid B, an active pharmaceutical compound present in Salvia miltiorrhiza, exerts a neuroprotective effect in animal models of brain and spinal cord injury. Salvianolic acid B can promote recovery of neurological function; however, its protective effect on the myelin sheath after spinal cord injury remains poorly understood. Thus, in this study, in vitro tests showed that salvianolic acid B contributed to oligodendrocyte precursor cell differentiation, and the most effective dose was 20 μg/mL. For in vivo investigation, rats with spinal cord injury were intraperitoneally injected with 20 mg/kg salvianolic acid B for 8 weeks. The amount of myelin sheath and the number of regenerating axons increased, neurological function recovered, and caspase-3 expression was decreased in the spinal cord of salvianolic acid B-treated animals compared with untreated control rats. These results indicate that salvianolic acid B can protect axons and the myelin sheath, and can promote the recovery of neurological function. Its mechanism of action is likely to be associated with inhibiting apoptosis and promoting the differentiation and maturation of oligodendrocyte precursor cells.

Key words: nerve regeneration, spinal cord injury, salvianolic acid B, oligodendrocytes, myelin sheath, neural regeneration