中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (6): 1001-1005.doi: 10.4103/1673-5374.184504

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

miR-148b-3p通过靶向Cand1可促进体外培养许旺细胞的迁移

  

  • 收稿日期:2015-12-22 出版日期:2016-06-30 发布日期:2016-06-30
  • 基金资助:

    国家重点基础研究计划资助项目(2014cb542202);863计划(2012aa020502);国家自然科学基金(81130080,81371389,81571198);中国南通大学自然科学基金(no.13040397);江苏高校优势学科建设项目(PAPD)

miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1

Tian-mei Qian#, Li-li Zhao#, Jing Wang, Ping Li, Jing Qin, Yi-sheng Liu, Bin Yu, Fei Ding, Xiao-song Gu, Song-lin Zhou*   

  1. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Co-innovation Center of Neuroregeneration, Nantong, Jiangsu Province, China
  • Received:2015-12-22 Online:2016-06-30 Published:2016-06-30
  • Contact: Song-lin Zhou, Ph.D., songlin.zhou@ntu.edu.cn.
  • Supported by:

    This study was supported by the National Key Basic Research Program of China, No. 2014CB542202; the National High-Tech R&D Program of China (863 Program), No. 2012AA020502; the National Natural Science Foundation of China, No. 81130080, 81371389 and 81571198; the Natural Science Foundation of Nantong University of China, No. 13040397; the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

摘要:

坐骨神经损伤后,许旺细胞可通过分化、成熟、增殖和迁移形成一个路径来引导轴突生长,以此促进损伤神经的修复。以往的一些芯片分析和功能筛选实验发现,坐骨神经损伤后许多miRNA的表达可发生改变,并影响许旺细胞的增殖和迁移。我们选择可促进乳腺癌细胞增殖的miR-148b-3p为对象,观察其对体外培养的许旺细胞迁移的影响,Transwell迁移小室实验发现,上调miR-148b-3p的表达能促进许旺细胞的迁移,而沉默miR-148b-3p之后,体外培养的许旺细胞的迁移被抑制。进一步采用萤光素酶双报告基因实验检测发现,Cullin-associated and neddylation-dissociated protein 1(Cand1)是miR-148b-3p的直接靶向基因,敲低Cand1基因的表达能够逆转miR-148b-3p抑制剂对许旺细胞迁移的抑制作用。结果证实,miR-148b-3p能够通过负调控Cand1来促进体外培养的许旺细胞的迁移。 

orcid: 0000-0001-8598-0922 (Songlin Zhou)

关键词: 神经再生, 坐骨神经损伤, miR-148b-3p, 许旺细胞, 迁移, Cand1, 基因表达, 基因芯片, 周围神经损伤, 机制

Abstract:

MicroRNAs (miRNAs) are small, non-coding RNAs that negatively adjust gene expression in multifarious biological processes. However, the regulatory effects of miRNAs on Schwann cells remain poorly understood. Previous microarray analysis results have shown that miRNA expression is altered following sciatic nerve transaction, thereby affecting proliferation and migration of Schwann cells. This study investigated whether miR-148b-3p could regulate migration of Schwann cells by directly targeting cullin-associated and neddylation-dissociated 1 (Cand1). Up-regulated expression of miR-148b-3p promoted Schwann cell migration, whereas silencing of miR-148b-3p inhibited Schwann cell migration in vitro. Further experiments confirmed that Cand1 was a direct target of miR-148b-3p, and Cand1 knockdown reversed suppression of the miR-148b-3p inhibitor on Schwann cell migration. These results suggested that miR-148b-3p promoted migration of Schwann cells by directly targeting Cand1 in vitro.

Key words: nerve regeneration, sciatic nerve injury, miR-148b-3p, Schwann cells, migration, Cand1, gene expression, microarray, peripheral nerve injury, mechanisms, neural regeneration