中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (6): 1006-1012.doi: 10.4103/1673-5374.184505

• 原著:视神经损伤修复保护与再生 • 上一篇    下一篇

嗅鞘细胞与睫状神经营养因子协同促进视神经损伤大鼠视神经修复的研究

  

  • 收稿日期:2015-12-22 出版日期:2016-06-30 发布日期:2016-06-30
  • 基金资助:

    全军重点课题(BJN14C001)

Synergetic effects of ciliary neurotrophic factor and olfactory ensheathing cells on optic nerve reparation (complete translation)

Dan-ping Yin1, #, Qing-ying Chen2, #, Lin Liu3, *   

  1. 1 Department of Disease Control and Prevention, Jinan Military General Hospital, Jinan, Shandong Province, China 2 Medical Department, Jinan Military General Hospital, Jinan, Shandong Province, China 3 Department of Ophthalmology, Renji Hospital Affiliated to Shanghai JiaoTong University School of Medicine, Shanghai, China
  • Received:2015-12-22 Online:2016-06-30 Published:2016-06-30
  • Contact: Lin Liu, linliu@sh163.net.
  • Supported by:

    This study was supported by the Dean Fund of Jinan Military General Hospital in 2015, No. 2015QN02.

摘要:

目前尚未找到显修复损伤视神经和改善其再生微环境的确切有效方法。有研究认为,玻璃体注射睫状神经营养因子及移植嗅鞘细胞对颅内段视神经损伤后神经纤维长距离再生有促进作用。为验证该结果,我们采用嗅鞘细胞混悬液浓缩后用微玻管注入视神经吸断伤模型大鼠神经损伤处,并与睫状神经营养因子玻璃体内注射联合应用。逆行示踪标记发现,视神经损伤后4周,单独移植嗅鞘细胞的大鼠视网膜神经节细胞存活数和神经元轴突密度优于单独睫状神经营养因子干预,嗅鞘细胞和睫状神经营养因子联合应用效果优于两者单独应用。结果证实嗅鞘细胞保护损伤视神经元的作用优于睫状神经营养因子,且两者联合干预能达到更好的修复效果。 

orcid: 0000-0002-0649-9688 (Lin Liu)

关键词: 神经再生, 视神经损伤, 修复, 再生, 嗅鞘细胞, 移植, 睫状神经营养因子, 视网膜神经节细胞, 细胞存活, 玻璃体内注射, 逆行标记

Abstract:

At present, there is no effective treatment for the repair of the optic nerve after injury, or improvement of its microenvironment for regeneration. Intravitreally injected ciliary neurotrophic factor (CNTF) and olfactory ensheathing cells (OECs) promote the long-distance regrowth of severed optic nerve fibers after intracranial injury. Here, we examined the efficacy of these techniques alone and in combination, in a rat model of optic nerve injury. We injected condensed OEC suspension at the site of injury, or CNTF into the vitreous body, or both simultaneously. Retrograde tracing techniques showed that 4 weeks postoperatively, the number of surviving retinal ganglion cells and their axonal density in the optic nerve were greater in rats subjected to OEC injection only than in those receiving CNTF injection only. Furthermore, combined OEC + CNTF injection achieved better results than either monotherapy. These findings confirm that OECs are better than CNTF at protecting injured neurons in the eye, but that combined OEC and CNTF therapy is notably more effective than either treatment alone.

Key words: nerve regeneration, optic nerve injury, repair, regeneration, olfactory ensheathing cells, ciliary neurotrophic factor, retinal ganglion cells, cell survival, intravitreal injection, retrograde tracing, neural regeneration