中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2017, Vol. 12 ›› Issue (3): 458-463.doi: 10.4103/1673-5374.202927

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

月见草油联合阈下电刺激促进挤压损伤坐骨神经的再生和功能恢复

  

  • 收稿日期:2017-02-03 出版日期:2017-03-15 发布日期:2017-03-15

Combination therapy using evening primrose oil and electrical stimulation to improve nerve function following a crush injury of sciatic nerve in male rats

Omid Badri 1, Parviz Shahabi2, Jalal Abdolalizadeh3, Mohammad Reza Alipour4, Hadi Veladi5, Mehdi Farhoudi2, Mohsen Sharif Zak6   

  1. 1 Tabriz University of Medical Sciences, International Branch Aras, Tabriz, Iran; 2 Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; 3 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; 4 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; 5 Microsystem Fabrication Laboratory, Tabriz University, Tabriz, Iran; 6 Department of Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  • Received:2017-02-03 Online:2017-03-15 Published:2017-03-15
  • Contact: Parviz Shahabi, Ph.D. in physiology, parvizshahabi@gmail.com.

PDF

731

摘要：

研究显示，月见草油中γ-亚麻油酸和亚麻油酸对维持神经正常结构有重要作用；电刺激可促进周围神经挤压伤后脑源性神经营养因子的表达和神经再生。为观察月见草油联合副作用较小的阈下电刺激对大鼠坐骨神经挤压伤后功能恢复的作用，实验首先用小号止血钳钳夹雄性大鼠坐骨神经建立坐骨神经挤压伤模型，然后给予该模型灌胃4周的月见草油和/或阈下电刺激（波宽0.1 ms，频率20Hz，强度 60 µA）干预。发现月见草油和/或电刺激干预可明显改善损伤坐骨神经功能。月见草油联合阈下电刺激干预使大鼠损伤坐骨神经P0免疫阳性的再髓鞘化轴突数量增加，再髓鞘化的轴突形态也较月见草油或阈下电刺激单独干预更接近正常轴突。月见草油联合阈下电刺激干预可明显减轻损伤侧肌肉萎缩程度，提高损伤坐骨神经传导速度。相对于两者单独应用，月见草油联合阈下电刺激可更好地刺激损伤坐骨神经轴突再髓鞘化，并改善其功能。

ORCID:0000-0001-5594-5544(Parviz Shahabi)

关键词: 神经再生, 周围神经损伤, 坐骨神经损伤, 月见草油, 电刺激, 坐骨神经指数, 卡肤电极

Abstract:

Peripheral nerve injuries with a poor prognosis are common. Evening primrose oil (EPO) has beneficial biological effects and immunomodulatory properties. Since electrical activity plays a major role in neural regeneration, the present study investigated the effects of electrical stimulation (ES), combined with evening primrose oil (EPO), on sciatic nerve function after a crush injury in rats. In anesthetized rats, the sciatic nerve was crushed using small haemostatic forceps followed by ES and/or EPO treatment for 4 weeks. Functional recovery of the sciatic nerve was assessed using the sciatic functional index. Histopathological changes of gastrocnemius muscle atrophy were investigated by light microscopy. Electrophysiological changes were assessed by the nerve conduction velocity of sciatic nerves. Immunohistochemistry was used to determine the remyelination of the sciatic nerve following the interventions. EPO + ES, EPO, and ES obviously improved sciatic nerve function assessed by the sciatic functional index and nerve conduction velocity of the sciatic nerve at 28 days after operation. Expression of the peripheral nerve remyelination marker, protein zero (P0), was increased in the treatment groups at 28 days after operation. Muscle atrophy severity was decreased significantly while the nerve conduction velocity was increased significantly in rats with sciatic nerve injury in the injury + EPO + ES group than in the EPO or ES group. Totally speaking, the combined use of EPO and ES may produce an improving effect on the function of sciatic nerves injured by a crush. The increased expression of P0 may have contributed to improving the functional effects of combination therapy with EPO and ES as well as the electrophysiological and histopathological features of the injured peripheral nerve.

Key words: nerve regeneration, peripheral nerve injury, sciatic nerve injury, evening primrose oil, electrical stimulation, sciatic functional index, cuff electrode, neural regeneration