中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (2): 440-449.doi: 10.4103/1673-5374.317986

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

嗅球与嗅粘膜来源嗅鞘细胞基因和蛋白表达谱的差异 

  

  • 出版日期:2022-02-15 发布日期:2021-10-08

Gene and protein expression profiles of olfactory ensheathing cells from olfactory bulb versus olfactory mucosa 

Yuan-Xiang Lan1, 2, 3, #, Ping Yang4, #, Zhong Zeng1, 2, 3, Neeraj Yadav3, 5, Li-Jian Zhang1, 2, 3, Li-Bin Wang6, *, He-Chun Xia2, 3, *   

  1. 1School of Clinical Medicine, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, China; 2Ningxia Human Stem Cell Institute, General Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, China; 3Department of Neurosurgery, General Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, China; 4Clinical Laboratory Center, General Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, China; 5School of International Education, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, China; 6Biochip Research Center, General Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, China
  • Online:2022-02-15 Published:2021-10-08
  • Contact: He-Chun Xia, xhechun@nyfy.com.cn; Li-Bin Wang, PhD, wanglibin007@126.com.
  • Supported by:
    This work was supported by Key Research Projects of Ningxia Hui Autonomous Region of China, No. 2018BCG01002 (to HCX) and the Natural Science Foundation of Ningxia Hui Autonomous Region of China, No. NZ17150 (to PY).

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摘要：

嗅球来源和嗅粘膜来源的嗅鞘细胞都具有神经修复功能，但尚不清楚两者治疗效果差异的原因。实验从小鼠的嗅球和嗅粘膜中分别提取了嗅鞘细胞，然后以转录组和蛋白质组测序技术来比较嗅球与嗅粘膜来源的嗅鞘细胞基因和蛋白表达的差异。（1）见2种嗅鞘细胞均高表达调节细胞生长、增殖、凋亡和血管内皮细胞再生的基因和蛋白。嗅球来源嗅鞘细胞的差异表达的基因和蛋白主要参与调节神经再生和轴突再生与延伸、神经冲动传导、轴突损伤反应等方面起着重要作用，而嗅粘膜来源嗅鞘细胞的差异表达基因和蛋白则主要参与炎症反应、防御反应、细胞因子结合、细胞迁移、伤口愈合调节等过程，具有更积极的调节作用；（2）结果提示这些差异表达的基因和蛋白可能是2种嗅鞘细胞表现出不同作用的机制。实验于2017年2月13日经宁夏医科大学总医院动物实验伦理委员会批准（批准号2017-073）。

https://orcid.org/0000-0002-9609-7809 (Yuan-Xiang Lan); https://orcid.org/0000-0002-7861-1560 (Li-Bin Wang); 
https://orcid.org/0000-0002-3058-8162 (He-Chun Xia)

关键词: 嗅鞘细胞, 嗅球, 嗅粘膜, 基因, 蛋白, 基因本体论, 京都基因与基因组百科全书, 分子功能, 细胞成分, 生物过程

Abstract: Olfactory ensheathing cells (OECs) from the olfactory bulb (OB) and the olfactory mucosa (OM) have the capacity to repair nerve injury. However, the difference in the therapeutic effect between OB-derived OECs and OM-derived OECs remains unclear. In this study, we extracted OECs from OB and OM and compared the gene and protein expression profiles of the cells using transcriptomics and non-quantitative proteomics techniques. The results revealed that both OB-derived OECs and OM-derived OECs highly expressed genes and proteins that regulate cell growth, proliferation, apoptosis and vascular endothelial cell regeneration. The differentially expressed genes and proteins of OB-derived OECs play a key role in regulation of nerve regeneration and axon regeneration and extension, transmission of nerve impulses and response to axon injury. The differentially expressed genes and proteins of OM-derived OECs mainly participate in the positive regulation of inflammatory response, defense response, cytokine binding, cell migration and wound healing. These findings suggest that differentially expressed genes and proteins may explain why OB-derived OECs and OM-derived OECs exhibit different therapeutic roles. This study was approved by the Animal Ethics Committee of the General Hospital of Ningxia Medical University (approval No. 2017-073) on February 13, 2017.

Key words: biological process, cellular component, gene, Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, molecular function, olfactory bulb, olfactory ensheathing cells, olfactory mucosa, protein

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