中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (24): 2213-2224.doi: 10.3969/j.issn.1673-5374.2013.24.001

• 原著:脊髓损伤修复保护与再生 •    下一篇

碱性成纤维细胞生长因子减缓受损脊髓运动终板的退变

  

  • 收稿日期:2013-04-04 修回日期:2013-06-08 出版日期:2013-08-25 发布日期:2013-08-25

Basic fibroblast growth factor attenuates the degeneration of injured spinal cord motor endplates

Jianlong Wang1, Jianfeng Sun2, Yongxiang Tang3, Gangwen Guo1, Xiaozhe Zhou1, Yanliang Chen1, Minren Shen1   

  1. 1 Department of Orthopedics, Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China
    2 Department of Orthopedics, Yichang Central People’s Hospital, Yichang 443003, Hubei Province, China
    3 Department of Nuclear Medicine, Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China
  • Received:2013-04-04 Revised:2013-06-08 Online:2013-08-25 Published:2013-08-25
  • Contact: Minren Shen, M.D., Department of Orthopedics, Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China, shenminren@163.com.
  • About author:Jianlong Wang, Associate professor, Master’s supervisor.

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摘要：

近年来研究表明，受损脊髓损伤远端及神经肌肉接头处，由于失去高位神经中枢的联系，同样会发生继发性退变和损害。实验采用改良Allen’s撞击法建立脊髓损伤大鼠模型，以蛛网膜下腔置管分多次局部注入碱性成纤维细胞生长因子溶液。结果显示，注入碱性成纤维细胞生长因子的脊髓损伤大鼠BBB评分较高，运动功能恢复更好；苏木精-伊红染色脊髓胶质瘢痕增生不明显，胫前肌肌纤维进行性萎缩较轻。胫前肌与脊髓降钙素基因相关肽和乙酰胆碱酯酶免疫组化染色吸光度值随时间变化趋势基本相同，注入碱性成纤维细胞生长因子后染色的吸光度值较高。结果说明，脊髓损伤后远端运动神经元及运动终板发生了退变，且脊髓前角运动神经元胞体内与运动终板内的降钙素基因相关肽和乙酰胆碱酯酶的变化趋势基本一致，碱性成纤维细胞生长因子通过减轻脊髓损伤后脊髓远端前角运动神经元内降钙素基因相关肽表达和使乙酰胆碱酯酶的下降而保护运动终板。

关键词: 神经再生, 脊髓损伤, 运动终板, 碱性成纤维细胞生长因子, 降钙素基因相关肽, 乙酰胆碱酯酶, 蛛网膜下腔置管, 基金资助文章

Abstract:

The distal end of the spinal cord and neuromuscular junction may develop secondary degeneration and damage following spinal cord injury because of the loss of neural connections. In this study, a rat model of spinal cord injury, established using a modified Allen’s method, was injected with basic fibroblast growth factor solution via subarachnoid catheter. After injection, rats with spinal cord injury displayed higher scores on the Basso, Beattie and Bresnahan locomotor scale. Motor function was also well recovered and hematoxylin-eosin staining showed that spinal glial scar hyperplasia was not apparent. Additionally, anterior tibial muscle fibers slowly, but progressively, atrophied. Immunohistochemical staining showed that the absorbance values of calcitonin gene related pep-tide and acetylcholinesterase in anterior tibial muscle and spinal cord were similar, and injection of basic fibroblast growth factor increased this absorbance. Results showed that after spinal cord injury, the distal motor neurons and motor endplate degenerated. Changes in calcitonin gene related pep-tide and acetylcholinesterase in the spinal cord anterior horn motor neurons and motor endplate then occurred that were consistent with this regeneration. Our findings indicate that basic fibroblast growth factor can protect the endplate through attenuating the decreased expression of calcitonin gene related peptide and acetylcholinesterase in anterior horn motor neurons of the injured spinal cord.

Key words: neural regeneration, spinal cord injury, motor endplate, basic fibroblast growth factor, calcitonin gene related peptide, acetylcholinesterase, subarachnoid catheter, grants-supported paper, neuroregeneration