中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (27): 2540-2547.doi: 10.3969/j.issn.1673-5374.2013.27.005

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

跑台步行训练提高不完全性脊髓损伤脊髓回路的可塑性

  

  • 收稿日期:2012-12-14 修回日期:2012-12-14 出版日期:2013-09-25 发布日期:2013-09-25
  • 基金资助:

    国家自然科学基金项目（30872604，81171862）

Treadmill step training promotes spinal cord neural plasticity after incomplete spinal cord injury

Tiansheng Sun1, Chaoqun Ye1, Jun Wu1, Zhicheng Zhang1, Yanhua Cai1, Feng Yue2   

  1. 1 Department of Orthopedics, Beijing Army General Hospital, Beijing 100700, China

    2 Department of Rehabilitation, Beijing Physical Education Institute, Beijing 100088, China
  • Received:2012-12-14 Revised:2012-12-14 Online:2013-09-25 Published:2013-09-25
  • Contact: Tiansheng Sun, Master, Professor, Department of Orthopedics, Beijing Army General Hospital, Beijing 100700, China, suntiansheng-@163.com.
  • Supported by:

    This work was sponsored by the National Natural Science Foundation of China, No. 30872604, 81171862.

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摘要：

大量证据表明，跑台步行训练可显著影响腰骶段脊髓完全横断大鼠脊髓通路，由于无法确定动物运动功能恢复是来自于跑台步行训练还是动物的自发性步行训练，所以对脊髓不完全损伤后跑台步行训练的效果仍有争论。为此，实验选第10胸椎段脊髓挫伤致不完全损伤1周的大鼠进行为期10周的跑台步行训练，5d/周，1次/d，每次15-30min，开始速度为3m/min，以后根据大鼠步行情况逐渐增加。结果显示，训练后3周大鼠后肢BBB评分明显增加，脊髓损伤组织周围生长相关蛋白43的表达量明显增加，且第二腰椎脊髓纤维周围神经元中酪氨酸羟化酶表达增加，损伤11周后脊髓病变部位的脑源性神经营养因子表达水平与模型组接近。提示跑台步行训练可明显促进不完全性脊髓损伤大鼠运动功能的恢复，促进脊髓损伤部位周围组织和损伤部位下段的神经可塑性。

关键词: 神经再生, 脊髓损伤, 神经康复, 不完全性脊髓损伤, 跑台步行训练, 脊髓可塑性, 生长相关蛋白43, 酪氨酸羟化酶, 运动功能恢复, 基金资助文章, 图片文章

Abstract:

A large body of evidence shows that spinal circuits are significantly affected by training, and that intrinsic circuits that drive locomotor tasks are located in lumbosacral spinal segments in rats with complete spinal cord transection. However, after incomplete lesions, the effect of treadmill training has been debated, which is likely because of the difficulty of separating spontaneous stepping from specific training-induced effects. In this study, rats with moderate spinal cord contusion were sub-jected to either step training on a treadmill or used in the model (control) group. The treadmill training began at day 7 post-injury and lasted 20 ± 10 minutes per day, 5 days per week for 10 weeks. The speed of the treadmill was set to 3 m/min and was increased on a daily basis according to the tolerance of each rat. After 3 weeks of step training, the step training group exhibited a sig-nificantly greater improvement in the Basso, Beattie and Bresnahan score than the model group. The expression of growth-associated protein-43 in the spinal cord lesion site and the number of tyrosine hydroxylase-positive ventral neurons in the second lumbar spinal segment were greater in the step training group than in the model group at 11 weeks post-injury, while the levels of brain-derived neurotrophic factor protein in the spinal cord lesion site showed no difference between the two groups. These results suggest that treadmill training significantly improves functional re-covery and neural plasticity after incomplete spinal cord injury.

Key words: neural regeneration, spinal cord injury, neurorehabilitation, incomplete spinal cord injury, treadmill training, spinal cord plasticity, growth-associated protein-43, tyrosine hydroxylase, function recovery, grants-supported paper, neuroregeneration