中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (3): 285-292.doi: 10.4103/1673-5374.128225

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

氨基化碳纳米管适合作为神经生长因子的载体?

  

  • 收稿日期:2013-10-17 出版日期:2014-02-15 发布日期:2014-02-15

Can amino-functionalized carbon nanotubes carry functional nerve growth factor?

Wen Chen1, 2, Qing Xiong2, Quanxia Ren2, Yake Guo2, Gao Li1   

  1. 1 School of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China
    2 School of Pharmacy, Shihezi University, Shihezi, Xinjiang Uygur Autonomous Reigion, China
  • Received:2013-10-17 Online:2014-02-15 Published:2014-02-15
  • Contact: Gao Li, M.D., School of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China, ligao-tj@163.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81160395.

摘要:

碳纳米管能载带蛋白进入细胞并发挥生物效应,且氨基化碳纳米管具有很好的溶解性、生物相容性以及较高的反应活性和较低的毒性,本身有促进神经细胞生长的功能。实验采用氨基功能化的多壁碳纳米管(MWCNTs-EDA)非共价接枝神经生长因子制备碳纳米管神经生长因子复合物(MWCNTs-NGF),考察所得复合物的理化性质、对嗜铬细胞瘤细胞(PC12细胞)和鸡胚背根神经节的细胞毒性以及其生物活性。结果显示,氨基功能化改善了碳纳米管神经生长因子复合物的分散性,降低了其对嗜铬细胞瘤细胞(PC12细胞)的毒性,且在促进PC12细胞分化及促进鸡胚背根神经节神经生长方面的作用更加明显。

关键词: 神经再生, 脊髓损伤, 碳纳米管, 氨基化, 神经生长因子, 细胞毒性, 生物活性, 分散性, PC12细胞, 鸡胚背根神经节, 国家自然科学基金

Abstract:

Carbon nanotubes can carry protein into cells to induce biological effects. Amino-functionalized carbon nanotubes are soluble and biocompatible, have high reactivity and low toxicity, and can help promote nerve cell growth. In this study, amino-functionalized ethylenediamine-treated multi-walled carbon nanotubes were used to prepare carbon nanotubes-nerve growth factor complexes by non-covalent grafting. The physicochemical properties, cytotoxicity to PC12 and chick embryo dorsal root ganglion, and biological activity of the carbon nanotubes-nerve growth factor complexes were investigated. The results showed that amino functionalization improved carbon nanotubes-nerve growth factor complex dispersibility, reduced their toxicity to PC12 cells, and promoted PC12 cell differentiation and chick embryo dorsal root ganglion.

Key words: nerve regeneration, spinal cord injury, carbon nanotubes, amino functionalization, ner-ve growth factor, cytotoxicity, biological activity, dispersibility, PC12 cells, chick embryo dorsal root ganglion, NSFC grant, neural regeneration