中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (8): 702-707.doi: 10.3969/j.issn.1673-5374.2013.08.004

• 原著:退行性病与再生 • 上一篇    下一篇

钙结合蛋白D28K参与雌激素治疗帕金森病

  

  • 收稿日期:2012-05-22 修回日期:2012-09-15 出版日期:2013-03-15 发布日期:2013-03-15

Role of calbindin-D28K in estrogen treatment for Parkinson’s disease

Chunhua Wang1, 2, 3, Chao Jiang1, Honghua Yuan1, Chenghua Xiao2, Dianshuai Gao1, 2   

  1. 1 Department of Neurobiology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China
    2 Department of Neurology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China
    3 Department of Neurology, Funing County People’s Hospital, Yancheng 224400, Jiangsu Province, China
  • Received:2012-05-22 Revised:2012-09-15 Online:2013-03-15 Published:2013-03-15
  • Contact: Dianshuai Gao, M.D., Professor, Department of Neurobiology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China; Department of Neurology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China, gds@xzmc.edu.cn.
  • About author:Chunhua Wang★, Master.

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摘要：

越来越多的研究表明，雌激素对黑质纹状体系统有神经保护作用。建立C57BL/6小鼠腹腔注射1-甲基-4-苯基-1,2,3,6-四氢吡啶的帕金森病模型，侧脑室注射17-β雌二醇连续14d。免疫荧光双标检测显示，雌激素增加帕金森病模型小鼠中脑黑质部位多巴胺能神经元中酪氨酸羟化酶和钙结合蛋白D28K的表达，且两者共表达也增加，行为学结果表明雌激素能改善帕金森病模型小鼠的行为。

关键词: 神经再生, 神经退行性变, 雌激素, 钙结合蛋白D28K, 帕金森病, 多巴胺能神经元, 酪氨酸羟化酶, 黑质, 图片文章

Abstract:

Studies have shown that estrogen has neuroprotective effects on the nigrostriatal system. The present study established a Parkinson’s disease model in C57BL/6 mice by intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrapyridine. The mice were subjected to 17β estradiol injection into the lateral ventricle. Immunofluorescence double staining showed that estrogen increased tyrosine hydroxylase and calbindin-D28K expression and co-expression in dopaminergic neurons of midbrain substantia nigra pars compacta of model mice. Behavior experiments showed that estrogen improved swimming and hanging behaviors in this mouse model of Parkinson’s disease.

Key words: neural regeneration, neurodegenerative diseases, estrogen, calbindin-D28K, Parkinson’s disease, dopaminergic neuron, tyrosine hydroxylase, photographs-containing paper, neuroregeneration