中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (12): 1103-1112.doi: 10.3969/j.issn.1673-5374.2013.12.005

• 原著:退行性病与再生 • 上一篇    下一篇

刺五加苷B/E改善衰老模型大鼠的学习记忆能力

  

  • 收稿日期:2012-12-03 修回日期:2013-03-09 出版日期:2013-04-25 发布日期:2013-04-25

Eleutheroside B or E enhances learning and memory in experimentally aged rats

Debin Huang, Zehua Hu, Zhaofen Yu   

  1. Department of Pharmacology, Hubei Institute for Nationalities, Enshi 445000, Hubei Province, China
  • Received:2012-12-03 Revised:2013-03-09 Online:2013-04-25 Published:2013-04-25
  • Contact: Debin Huang★, Master, Professor, Department of Pharmacology, Hubei Institute for Nationalities, Enshi 445000, Hubei Province, China, hdb66910@163.com.
  • Supported by:

    湖北省教育部基金（D20111903）

PDF

1000

摘要：

刺五加主要成分为刺五加苷B/E，具有抗疲劳、增强记忆、改善人脑智力活动的作用。有研究表明应用大剂量刺五加注射液能显著缓解阿尔茨海默病患者的临床症状、延缓病程发展。鉴于此，喹啉酸定位注射大鼠海马CA1区制作衰老大鼠模型，将低中高剂量刺五加苷B/E(50，100，200 mg/kg)对模型大鼠进行腹腔注射，并设石杉碱甲组、模型组作对照。给药4周后，行为学检测发现，中高剂量刺五加苷B/E干预的大鼠寻找Morris水迷宫平台潜伏期和错误次数呈剂量依赖性减少。苏木精-伊红染色结果显示，与模型组相比，刺五加苷B/E各组的存活海马神经细胞数较多，病理损伤较轻。海马匀浆液检测发现，刺五加苷B/E各剂量组胆碱乙酰化酶活性均升高，乙酰胆碱含量呈剂量依赖性升高，胆碱含量呈剂量依赖性逐渐降低，与石杉碱甲组相近。结果证实，刺五加苷B/E可改善衰老大鼠的学习记忆能力，其机制可能与刺五加苷B/E活化海马神经细胞胆碱乙酰化酶，或促进胆碱再利用，而加速乙酰胆碱的合成有关。

关键词: 神经再生, 中医药, 刺五加苷B/E, 喹啉酸, 衰老大鼠, 石杉碱甲, 学习记忆, 海马, 乙酰胆碱, 胆碱酯酶, 胆碱, 机制, 基金资助文章

Abstract:

Eleutheroside B or E, the main component of Acanthopanax, can relieve fatigue, enhance memory, and improve human cognition. Numerous studies have confirmed that high doses of acetylcholine significantly attenuate clinical symptoms and delay the progression of Alzheimer’s disease. The present study replicated a rat model of aging induced by injecting quinolinic acid into the hippocampal CA1 region. These rats were intraperitoneally injected with low, medium and high doses of eleutheroside B or E (50, 100, 200 mg/kg), and rats injected with Huperzine A or PBS were used as controls. At 4 weeks after administration, behavioral tests showed that the escape latencies and errors in searching for the platform in a Morris water maze were dose-dependently reduced in rats treated with medium and high-dose eleutheroside B or E. Hematoxylin-eosin staining showed that the number of surviving hippocampal neurons was greater and pathological injury was milder in three eleutheroside B or E groups compared with model group. Hippocampal homogenates showed enhanced cholinesterase activity, and dose-dependent increases in acetylcholine content and decreases in choline content following eleutheroside B or E treatment, similar to those seen in the Huperzine A group. These findings indicate that eleutheroside B or E improves learning and memory in aged rats. These effects of eleutheroside B or E may be mediated by activation of cholinesterase or enhanced reuse of choline to accelerate the synthesis of acetylcholine in hippocampal neurons.

Key words: neural regeneration, traditional Chinese medicine, eleutheroside B or E, quinolinic acid, aged rats, Huperzine A, learning and memory, hippocampus, acetylcholine, cholinesterase, choline, mechanism, grants-supported paper, neuroregeneration