中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (12): 1113-1121.doi: 10.3969/j.issn.1673-5374.2013.12.006

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

传统中药加味补肝汤是如何治疗糖尿病周围神经病变的？

  

  • 收稿日期:2012-12-05 修回日期:2013-03-27 出版日期:2013-04-25 发布日期:2013-04-25

Protective effect of Jiaweibugan decoction against diabetic peripheral neuropathy

Yu Wang1, Zeqi Chen1, Renqun Ye2, Yulei He1, Yuhong Li1, Xinjian Qiu1   

  1. 1 Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China
    2 Department of Internal Medicine, Chinese Medical Hospital in Bao’an District of Shenzhen City, Shenzhen 518133, Guangdong Province, China
  • Received:2012-12-05 Revised:2013-03-27 Online:2013-04-25 Published:2013-04-25
  • Contact: Zeqi Chen, Chief physician, Professor, Doctoral supervisor, Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China, czq4471680@yahoo.com.cn.
  • About author:Yu Wang☆, Studying for doctorate.
  • Supported by:

    湖南省卫生厅中医药科研基金资助项目(No. 06202)

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摘要：

氧自由基损伤被认为是糖尿病神经病变发生的直接或间接共同通路。为此，我们假设传统中药加味补肝汤可通过抗氧化应激途径起到治疗糖尿病神经病变的作用。实验采用腹腔注射链脲佐菌素法复制糖尿病大鼠模型，采用中药方剂加味补肝汤灌胃。运用比色法测定大鼠血清中氧化应激间接指标--丙二醛含量及谷胱甘肽水平，反转录聚合酶链式反应及免疫组织化学方法检测大鼠脊髓S4-6节段背根神经节氧化应激相关因子--核因子-κBP65及p38-分裂原活化蛋白激酶的表达。结果显示，加味补肝汤能明显改善糖尿病大鼠坐骨神经传导速度，下调血清丙二醛及脊髓S4-6节段背根神经节核因子-κBP65、p38-分裂原活化蛋白激酶的表达，并上调血清谷胱甘肽水平。证明中药方剂加味补肝汤能减轻糖尿病周围神经病变过程中的氧化应激反应，以此对损伤的周围神经发挥保护效应。

关键词: 神经再生, 中医药, 周围神经损伤, 加味补肝汤, 糖尿病周围神经病变, 丙二醛, 谷胱甘肽, 细胞核因子-κBP65, p38-分裂原活化蛋白激酶, 氧化应激, 牛磺酸, 链脲佐菌素, 氧自由基损伤, 基金资助文章

Abstract:

Oxygen free radical damage is regarded as a direct or indirect common pathway associated with diabetic neuropathy and is the main cause of complications in peripheral neuropathies. We speculate that Jiaweibugan decoction has a significant effect in treating diabetic peripheral neuropathy through an anti-oxidative stress pathway. In this study, a diabetic rat model was established by intraperitoneal injection of streptozotocin. Rats were treated with Jiaweibugan decoction via intragastric administration. The levels of malondialdehyde and glutathione, which are indirect indexes of oxidative stress, in serum were determined using a colorimetric method. The expression levels of nuclear factor kappa B p65 mRNA and p38 mitogen-activated protein kinase, which are oxidative stress associated factors, in the dorsal root ganglion of spinal S4–6 segments were evaluated by reverse-transcriptase polymerase chain reaction and immunohistochemistry. Results showed that, Jiaweibugan decoction significantly ameliorated motor nerve conduction velocity in diabetic rats, effectively decreased malondialdehyde levels in serum and the expression of nuclear factor kappa B p65 mRNA and p38 mitogen-activated protein kinase mRNA in the dorsal root ganglion, and increased glutathione levels in serum. Therefore, our experimental findings indicate that Jiaweibugan decoction plays an anti-oxidative stress role in the diabetic peripheral neuropathy process, which has a protective effect on peripheral nerve injury.

Key words: neural regeneration, traditional Chinese medicine, peripheral nerve injury, Jiaweibugan decoction, diabetic peripheral neuropathy, malondialdehyde, glutathione, nuclear factor kappa B p65, p38 mitogen-activated protein kinase, oxidative stress, taurine, streptozotocin, oxygen free radical damage, grants-supported paper, neuroregeneration