中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (22): 2039-2049.doi: 10.3969/j.issn.1673-5374.2013.22.003

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

破血化瘀复方中药治疗脑出血：上调神经营养因子的表达

  

  • 收稿日期:2013-04-12 修回日期:2013-07-08 出版日期:2013-08-05 发布日期:2013-08-05

Poxue Huayu and Tianjing Busui Decoction for cerebral hemorrhage

Jixiang Ren1, Xiangyu Zhou2, Jian Wang1, Jianjun Zhao1, Pengguo Zhang3   

  1. 1 Department of Encephalopathy, the Affiliated Hospital to Changchun University of Chinese Medicine, Changchun 130021, Jilin Province, China
    2 Grade 2010 Clinical Medicine Major, School of Clinical Medicine, Yanbian University, Yanji 133002, Jilin Province, China
    3 Department of Imaging, School of Second Clinical Medicine, Jilin University, Changchun 130041, Jilin Province, China
  • Received:2013-04-12 Revised:2013-07-08 Online:2013-08-05 Published:2013-08-05
  • Contact: Jianjun Zhao, Master, Professor, Doctoral supervisor, Department of Encephalopathy, the Affiliated Hospital to Changchun University of Chinese Medicine, Changchun 130021, Jilin Province, China, zhaojianjun8777@163.com. Pengguo Zhang, M.D., Associate professor, Master’s supervisor, Department of Imaging, School of Second Clinical Medicine, Jilin University, Changchun 130041, Jilin Province, China, Zhangyangren@126.com.
  • About author:Jixiang Ren, M.D., Attending physician. Jixiang Ren and Xiangyu Zhou contributed equally to this article.
  • Supported by:

    国家中药研究基地（中风病）建设项目；吉林省自然科学基金项目（201015211）。

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摘要：

实验以注射抗凝自体血方法建立脑出血大鼠模型，以5，10，20g/kg由水蛭、生大黄、生蒲黄、虻虫、瓜蒌、三七、石菖蒲、龟板胶为主要成分组成的“破血化瘀，填精补髓”中药汤剂灌胃治疗，1次/d，连续给药14d。结果显示治疗结束后脑出血大鼠脑含水量明显降低，脑血肿体积明显缩小，且免疫组化检测显示血肿周围组织脑源性神经营养因子、酪氨酸激酶B及血管内皮生长因子表达显著增加，同时反转录PCR检测证实血肿周围组织脑源性神经营养因子和酪氨酸激酶BmRNA表达显著增加，神经功能缺损明显减轻，提示“破血化瘀”中药汤剂可能通过上调脑内神经营养因子的表达治疗脑出血。

关键词: 神经再生, 中医药, 脑出血, 脑源性神经营养因子, 酪氨酸激酶B, 血管内皮生长因子, 破血化瘀, 填精补髓, 基金资助文章

Abstract:

This study established a rat model of cerebral hemorrhage by injecting autologous anticoagulated blood. Rat models were intragastrically administered 5, 10, 20 g/kg Poxue Huayu and Tianjing Busui Decoction, supplemented with Hirudo, raw rhubarb, raw Pollen Typhae, gadfly, Fructrs Trichosanthis, Radix Notoginseng, Rhizoma Acori Talarinowii, and glue of tortoise plastron, once a day, for 14 consecutive days. Results demonstrated that brain water content significantly reduced in rats with cerebral hemorrhage, and intracerebral hematoma volume markedly reduced after treat-ment. Immunohistochemical staining revealed that brain-derived neurotrophic factor, tyrosine ki-nase B and vascular endothelial growth factor expression noticeably increased around the sur-rounding hematoma. Reverse transcription-PCR revealed that brain-derived neurotrophic factor and tyrosine kinase B mRNA expression significantly increased around the surrounding hematoma. Neurologic impairment obviously reduced. These results indicated that Poxue Huayu and Tianjing Busui Decoction exert therapeutic effects on cerebral hemorrhage by upregulating the expression of brain-derived neurotrophic factor.

Key words: neural regeneration, traditional Chinese medicine, cerebral hemorrhage, brain-derived neurotrophic factor, tyrosine kinase B, vascular endothelial growth factor, Poxue Huayu and Tianjing Busui, grants-supported paper, neuroregeneration