中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (23): 2126-2133.doi: 10.3969/j.issn.1673-5374.2013.23.002

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

过长时间七氟醚预处理不能对永久性脑缺血产生神经保护

  

  • 收稿日期:2013-04-07 修回日期:2013-07-15 出版日期:2013-08-15 发布日期:2013-08-15

Is longer sevoflurane preconditioning neuroprotective in permanent focal cerebral ischemia

Caiwei Qiu1, 2, Bo Sheng3, Shurong Wang4, Jin Liu2   

  1. 1 West China School of Pharmacy, Sichuan University, Chengdu 610041, Sichuan Province, China
    2 Neuroscience Research Center, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
    3 Department of Anesthesiology, Panzhihua Central Hospital, Panzhihua 617000, Sichuan Province, China
    4 Department of Neurology, the 452 People’s Liberation Army Hospital, Chengdu 610041, Sichuan Province, China
  • Received:2013-04-07 Revised:2013-07-15 Online:2013-08-15 Published:2013-08-15
  • Contact: Jin Liu, M.D., Neuroscience Research Center, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China, scujinliu@gmail.com.
  • About author:Caiwei Qiu, Ph.D.

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摘要：

七氟醚预处理对脑缺血再灌注损伤具有神经保护作用，但对永久性脑缺血的效果还不确定。实验以电凝法建立永久性脑缺血大鼠模型前30，60，120min分别使大鼠预先吸入七氟醚。发现脑缺血前30，60min七氟醚进行了预处理均能显著减小永久性脑缺血24h大鼠的脑梗死体积，且缺血前60min七氟醚进行了预处理还能减少永久性脑缺血大鼠脑缺血半暗带中TUNEL和caspase-3阳性细胞数量，而延长七氟醚预处理至120min的大鼠却没有明显的神经保护作用。且缺血前60min七氟醚预处理，使脑缺血4d的永久性脑缺血大鼠神经行为学缺陷明显减轻，同时脑梗死体积减小。提示缺血前60min进行的七氟醚预处理是对永久性脑缺血大鼠产生神经保护作用的最优选择，且七氟醚预处理的神经保护效应可能与抑制凋亡和caspase-3的表达相关。

关键词: 神经再生, 脑损伤, 麻醉, 七氟醚, 预处理, 脑缺血, 凋亡, caspase-3

Abstract:

Sevoflurane preconditioning has neuroprotective effects in the cerebral ischemia/reperfusion model. However, its influence on permanent cerebral ischemia remains unclear. In the present study, the rats were exposed to sevoflurane for 15, 30, 60, and 120 minutes, followed by induction of perma-nent cerebral ischemia. Results demonstrated that 30- and 60-minute sevoflurane preconditioning significantly reduced the infarct volume at 24 hours after cerebral ischemia, and 60-minute sevoflurane preconditioning additionally reduced the number of TUNEL- and caspase-3-positive cells in the ischemic penumbra. However, 120-minute sevoflurane preconditioning did not show evident neuroprotective effects. Moreover, 60-minute sevoflurane preconditioning significantly at-tenuated neurological deficits and infarct volume in rats at 4 days after cerebral ischemia. These findings indicated that 60-minute sevoflurane preconditioning can induce the best neuroprotective effects in rats with permanent cerebral ischemia through the inhibition of apoptosis.

Key words: neural regeneration, brain injury, anesthesia, sevoflurane, preconditioning, cerebral ischemia, apoptosis, caspase-3, neuroregeneration