中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (25): 2343-2349.doi: 10.3969/j.issn.1673-5374.2013.25.005

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

传统中药丹红注射液维持了缺血再灌注损伤脑组织高尔基体的结构稳定

  

  • 收稿日期:2013-05-13 修回日期:2013-07-10 出版日期:2013-09-05 发布日期:2013-09-05

Danhong injection A modulator for Golgi structural stability after cerebral ischemia- reperfusion injury

Yan Wang, Zhiping Hu, Wei Lu   

  1. Department of Neurology, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China
  • Received:2013-05-13 Revised:2013-07-10 Online:2013-09-05 Published:2013-09-05
  • Contact: Zhiping Hu, Doctoral supervisor, Professor, Department of Neurology, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China, huzhipingxy@gmail.com. Wei Lu, Master’s supervisor, Associate professor, Department of Neurology, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China, luwei0338@sina.com.
  • About author:Yan Wang, Master.

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摘要：

实验采用线栓法制备脑缺血再灌注模型，并于造模前1d给大鼠腹腔注射8mL/kg丹红注射液，再灌注后6，24，48，72h和7d取大鼠脑组织进行观察。免疫组化染色结果显示，脑缺血再灌注大鼠海马转化生长因子β1表达增高，高尔基体形态结构蛋白GM130表达降低，而丹红注射液能明显提高转化生长因子β1和GM130的表达，并于再灌注后7d达到最高。实验发现给予丹红注射液的脑缺血再灌注大鼠前脑皮质高尔基体结构均未见明显改变，而未给予丹红注射液干预的脑缺血再灌注7d的大鼠前脑皮质神经元中可见部分高尔基体形态破坏。提示丹红注射液可通过提高脑缺血再灌注损伤大鼠脑组织转化生长因子β1和GM130的表达，维持神经元中高尔基体的稳定性，发挥神经保护作用。

关键词: 神经再生, 中医药, 丹红注射液, 脑缺血再灌注, 转化生长因子β1, 高尔基体, 基质蛋白, GM130, 脑损伤, 神经保护, 基金资助文章

Abstract:

The cerebral ischemia-reperfusion model was established using the suture occlusion method, and rats were intraperitoneally given 8 mL/kg Danhong injection once a day prior to model establishment. Rat brain tissues were harvested at 6, 24, 48, 72 hours after reperfusion. Immunohistochemical staining showed that transforming growth factor-β1 expression increased, while Golgi matrix protein GM130 expression decreased after cerebral ischemia-reperfusion. Danhong injection was shown to significantly up-regulate the expression of transforming growth factor-β1 and GM130, and expres-sion levels peaked at 7 days after reperfusion. At 7 days after cerebral ischemia-reperfusion, Golgi morphology was damaged in untreated rats, while Golgi morphology breakage was not observed after intervention with Danhong injection. These experimental findings indicate that Danhong injec-tion can up-regulate the expression of transforming growth factor-β1 and GM130, and maintain Golgi stability, thus playing a neuroprotective role in rats after cerebral ischemia-reperfusion.