中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (36): 3365-3372.doi: 10.3969/j.issn.1673-5374.2013.36.001

• 原著:脊髓损伤修复保护与再生 •    下一篇

大鼠毛囊干细胞的神经分化及修复脊髓损伤

  

  • 收稿日期:2013-09-25 修回日期:2013-11-09 出版日期:2013-12-25 发布日期:2013-12-25
  • 基金资助:

    伊朗Tehran 医科大学基金 (Tehran– Iran, No. 531)

Rat hair follicle stem cells differentiate and promote recovery following spinal cord injury

Nowruz Najafzadeh1, Maliheh Nobakht2, 3, 4, Bagher Pourheydar5, 6, Mohammad Ghasem Golmohammadi1   

  1. 1 Department of Anatomy and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
    2 Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
    3 Antimicrobial Resistance Research Center, Iran University of Medical Sciences, Tehran, Iran
    4 Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
    5 Department of Anatomical Sciences, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran
    6 Neurophysiology Research Center, Urmia University of Medical Sciences, Urmia, Iran
  • Received:2013-09-25 Revised:2013-11-09 Online:2013-12-25 Published:2013-12-25
  • Contact: Nowruz Najafzadeh, Ph.D., Assistant professor, Department of Anatomy and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran, n.najafzade@ arums.ac.ir.
  • Supported by:

    This study was financially supported by a grant from Iran University of Medical Sciences (Tehran–Iran), No. 531.

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摘要：

研究已证实，毛囊干细胞在体外可分化为神经元和胶质细胞，且可在体内修复脊髓损伤。实验分离和培养了大鼠触须的毛囊干细胞。将BrdU标记的毛囊干细胞注射于脊髓压迫损伤中心和距损伤中心2mm部位。免疫组化染色结果显示，细胞移植后3周，毛囊干细胞可在移植部位存活，并分化为少突胶质细胞和神经元样细胞。BBB评分评估显示，细胞移植后8周，毛囊干细胞移植明显改善脊髓损伤大鼠后肢运动功能。实验结果证实，移植毛囊干细胞可长期存活于脊髓损伤部位，并分化为神经元样细胞，从而改善脊髓损伤大鼠运动功能。

中国神经再生研究（英文版）杂志出版内容重点： 脑损伤；脊髓损伤；周围神经损伤；帕金森；神经影像；神经再生

关键词: 神经再生, 脊髓损伤, 细胞移植, 细胞疗法, 毛囊干细胞, 少突胶质细胞, 神经细胞, 受体相互作用蛋白, 基金资助文章

Abstract:

Emerging studies of treating spinal cord injury (SCI) with adult stem cells led us to evaluate the effects of transplantation of hair follicle stem cells in rats with a compression-induced spinal cord lesion. Here, we proposed a hypothesis that rat hair follicle stem cell transplantation can promote the recovery of injured spinal cord. Compression-induced spinal cord injury was induced in Wistar rats in this study. The bulge area of the rat vibrissa follicles was isolated, cultivated and characterized with nestin as a stem cell marker. 5-Bromo-2′-deoxyuridine (BrdU) labeled bulge stem cells were transplanted into rats with spinal cord injury. Immunohistochemical staining results showed that some of the grafted cells could survive and differentiate into oligodendrocytes (receptor-interacting protein positive cells) and neuronal-like cells (βIII-tubulin positive cells) at 3 weeks after transplantation. In addition, recovery of hind limb locomotor function in spinal cord injury rats at 8 weeks following cell transplantation was assessed using the Basso, Beattie and Bresnahan (BBB) locomotor rating scale. The results demon-strate that the grafted hair follicle stem cells can survive for a long time period in vivo and differentiate into neuronal- and glial-like cells. These results suggest that hair follicle stem cells can promote the recovery of spinal cord injury.

Key words: neural regeneration, spinal cord injury, cell transplantation, cell therapy, hair follicle stem cells, oligodendrocytes, nerve cells, glial cells, receptor-interacting protein, grants-supported paper, neuroregeneration