中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (1): 61-68.doi: 10.4103/1673-5374.125331

• 原著:退行性病与再生 • 上一篇    下一篇

基于脑白质损伤探讨开心解郁方治疗血管性抑郁的途径

  

  • 收稿日期:2013-10-20 出版日期:2014-01-05 发布日期:2014-01-05
  • 基金资助:

    国家自然科学基金(30672696;81072801)和北京市自然科学基金(7093129)。

Mechanism underlying the protective effect of Kaixin Jieyu Fang on vascular depression following cerebral white matter damage

Ying Zhang, Shijing Huang, Yanyun Wang, Junhua Pan, Jun Zheng, Xianhui Zhang, Yuxia Chen, Duojiao Li   

  1. Guang’anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, China
  • Received:2013-10-20 Online:2014-01-05 Published:2014-01-05
  • Contact: Shijing Huang, M.D., Guang’anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China, gamhsj@126.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 30672696, 81072801; and the Natural Science Foundation of Beijing in China, No. 7093129.

摘要:

中药开心解郁方可治疗血管性抑郁,但其抗抑郁的机制目前还不清楚。实验用双侧颈总动脉结扎法制作慢性脑缺血白质损害大鼠模型,之后每天用开心解郁方生粉混悬液灌胃进行干预。经开心解郁方治疗3,7,21d的脑缺血模型大鼠脑白质受损程度减轻,脑组织中Bcl-2蛋白及mRNA表达增加,Bax蛋白及mRNA表达下降。表明开心解郁方可减轻脑白质损伤,其作用机制与调控Bcl-2/Bax蛋白及其基因表达有关,这可能是该方剂治疗血管性抑郁的作用机制之一。

关键词: 神经再生, 中医药, 脑损伤, 开心解郁方, 血管性抑郁, 脑白质损伤, 病理形态, 脑缺血, 脱髓鞘, 双侧颈总动脉结扎, Bcl-2, Bax

Abstract:

The Chinese compound Kaixin Jieyu Fang can be used to treat vascular depression; however, the underlying mechanism remains unclear. This study established a rat model of chronic cerebral ischemia-caused white matter damage by ligation of the bilateral common carotid arteries. Rats received daily intragastric administration of a suspension of Kaixin Jieyu Fang powder. After 3, 7 and 21 days of treatment, the degree of white matter damage in the cerebral ischemia rat model was alleviated, Bcl-2 protein and mRNA expression in brain tissue increased, and Bax protein and mRNA expression decreased. These results indicate that Kaixin Jieyu Fang can alleviate cerebral white matter damage, and the underlying mechanism is associated with regulation of Bcl-2/Bax protein and mRNA expression, which is one of possible mechanism behind the protective effect of Kaixin Jieyu Fang against vascular depression.

Key words: nerve regeneration, vascular depression, ligation of the bilateral common carotid arteries, chronic cerebral ischemia, white matter damage, Kaixin Jieyu Fang powder, Bcl-2, Bax, NSFC grant, neural regeneration