中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (1): 76-83.doi: 10.4103/1673-5374.125333

• 原著:退行性病与再生 • 上一篇    下一篇

电针抑郁大鼠百会和印堂穴后海马基因表达的变化

  

  • 收稿日期:2013-11-20 出版日期:2014-01-05 发布日期:2014-01-05
  • 基金资助:

    国家自然科学基金面上项目：（81273847）

Hippocampal gene expression in a rat model of depression after electroacupuncture at the Baihui and Yintang acupoints

Dongmei Duan1, Xiuyan Yang2, Ya Tu3, Liping Chen1   

  1. 1 Department of Traditional Chinese Medicine of South Building, Chinese PLA General Hospital, Beijing, China
    2 Institute of Health Maintenance, Beijing University of Chinese Medicine, Beijing, China
    3 School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, Beijing, China
  • Received:2013-11-20 Online:2014-01-05 Published:2014-01-05
  • Contact: Dongmei Duan, M.D., Department of Traditional Chinese Medicine of South Building, Chinese PLA General Hospital, Beijing 100853, China, dongmei-d@hotmail.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81273847.

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摘要：

前期基础与临床研究已证实电针抗抑郁疗效确切，但对电针抗抑郁机制的探索大多仍停留在单个基因水平, 尚无全基因组分子水平的相关基因机制研究。实验采用大鼠全基因组基因芯片技术，从微观整体上研究电针后大鼠海马基因表达谱的变化，以及治疗结束后各组大鼠海马基因的表达。结果显示电针能改善抑郁模型大鼠行为活动，基因芯片检测显示电针百会、印堂穴治疗抑郁症的机制可能与22条基因表达调控有关，对其中与抑郁可能相关的VGF，Igf2，Tmp32，Loc500373，Hif1a，Folr1，Nmb和Rtn基因进行实时PCR 验证，这些基因均为在抑郁症中发生上调或下调变化，经电针治疗后上述基因的表达趋于正常。结果说明电针治疗抑郁症的作用与调控多条基因表达有关。

关键词: 神经再生, 针灸, 中医药, 抑郁症, 基因表达谱, 基因芯片, 电针, 百会, 印堂, 慢性应激, 行为学, 基金资助文章

Abstract:

Preliminary basic research and clinical findings have demonstrated that electroacupuncture therapy exhibits positive effects in ameliorating depression. However, most studies of the underlying mechanism are at the single gene level; there are few reports regarding the mechanism at the whole-genome level. Using a rat genomic gene-chip, we profiled hippocampal gene expression changes in rats after electroacupuncture therapy. Electroacupuncture therapy alleviated depression-related manifestations in the model rats. Using gene-chip analysis, we demonstrated that electroacupuncture at Baihui (DU20) and Yintang (EX-HN3) regulates the expression of 21 genes. Real-time PCR showed that the genes Vgf, Igf2, Tmp32, Loc500373, Hif1a, Folr1, Nmb, and Rtn were upregulated or downregulated in depression and that their expression tended to normalize after electroacupuncture therapy. These results indicate that electroacupuncture at Baihui  and Yintang modulates depression by regulating the expression of particular genes.

Key words: nerve regeneration, acupuncture, traditional Chinese medicine, depression, gene expression profiling, gene-chip, electroacupuncture, Baihui (DU20), Yintang (EX-HN3), chronic stress, behavior, NSFC grant, neural regeneration