中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (8): 798-805.doi: 10.4103/1673-5374.131596

• 原著:退行性病与再生 • 上一篇    下一篇

脂肪干细胞移植可促进阿尔茨海默病小鼠脑部新生神经元增殖

  

  • 收稿日期:2014-03-24 出版日期:2014-04-25 发布日期:2014-04-25
  • 基金资助:

    国家高技术研究发展计划(863计划)(2012AA020905);清华大学-裕元医学基金资助项目(20240000514)

Adipose-derived mesenchymal stem cell transplantation promotes adult neurogenesis in the brains of Alzheimer’s disease mice

Yufang Yan 1, Tuo Ma 1, Kai Gong 1, Qiang Ao 2, Xiufang Zhang 1, Yandao Gong 1   

  1. 1 State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Life Sciences, Tsinghua University, Beijing, China
    2 Institute of Neurological Disorders, Yuquan Hospital, Tsinghua University, Beijing, China
  • Received:2014-03-24 Online:2014-04-25 Published:2014-04-25
  • Contact: Yandao Gong, State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Life Sciences, Tsinghua University, Beijing 100084, China, gongyd@tsinghua.edu.cn.
  • Supported by:

    This work was supported by the National High-Tech Research and Development Program of China (863 Program), No. 2012AA020905; Tsinghua-Yue-Yuen Medical Sciences Fund, No. 20240000514.

摘要:

实验在APP/PS1转基因阿尔茨海默病模型小鼠海马区植入脂肪干细胞。免疫荧光染色结果表明,脂肪干细胞移植使APP/PS1小鼠脑内海马齿状回区域BrdU阳性的新生细胞数量显著升高,其中齿状回颗粒下区脂肪干细胞移植使得新生细胞中的成神经细胞(BrdU和DCX双阳性)的比例得到显著的提升。与此同时,脂肪干细胞移植也显著地增强了APP/PS1转基因阿尔茨海默病模型小鼠脑内室管膜下区域的神经发生过程。因此,我们认为海马内移植脂肪干细胞可以促进颗粒下和脑内室管膜下区域的神经发生过程,进而改善APP/PS1转基因阿尔茨海默病模型小鼠的认知功能。
 

关键词: 神经再生, 干细胞, 阿尔茨海默病, 脂肪干细胞, 细胞移植, 认知损伤, 氧化损伤, 神经发生, 基金资助文章

Abstract:

In the present study, we transplanted adipose-derived mesenchymal stem cells into the hippocampi of APP/PS1 transgenic Alzheimer’s disease model mice. Immunofluorescence staining revealed that the number of newly generated (BrdU+) cells in the subgranular zone of the dentate gyrus in the hippocampus was significantly higher in Alzheimer’s disease mice after adipose-derived mesenchymal stem cell transplantation, and there was also a significant increase in the number of BrdU+/DCX+ neuroblasts in these animals. Adipose-derived mesenchymal stem cell transplantation enhanced neurogenic activity in the subventricular zone as well. Furthermore, adipose-derived mesenchymal stem cell transplantation reduced oxidative stress and alleviated cognitive impairment in the mice. Based on these findings, we propose that adipose-derived mesenchymal stem cell transplantation enhances endogenous neurogenesis in both the subgranular and subventricular zones in APP/PS1 transgenic Alzheimer’s disease mice, thereby facilitating functional recovery.

Key words: nerve regeneration, stem cells, Alzheimer’s disease, adipose-derived mesenchymal stem cells, cell transplantation, cognitive impairment, oxidative stress, neurogenesis, 863 Program, neural regeneration