中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (8): 821-827.doi: 10.4103/1673-5374.131598

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

高浓度丝裂霉素C对周围神经功能存在潜在风险

  

  • 收稿日期:2014-03-21 出版日期:2014-04-25 发布日期:2014-04-25
  • 基金资助:

    国家自然科学基金面上项目（81171694,81201374, 81371968, 81371969）；江苏省高校前沿项目；江苏省自然科学基金项目（BK2012718, BK2011844）

Potential risk of mitomycin C at high concentrations on peripheral nerve structure

Tao Sui 1, Jinhong Zhang 2, Shihao Du 1, Changhui Su 3, Jun Que 4, Xiaojian Cao 1   

  1. 1 Department of Orthopedics, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China
    2 Department of Orthopedics, the Second Hospital of Nanjing, Affiliated to Southeast University, Nanjing, Jiangsu Province, China
    3 Department of Orthopedics, Affiliated Hospital of Taishan Medical College, Taishan, Shandong Province, China
    4 Department of Intensive Care Unit, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China
  • Received:2014-03-21 Online:2014-04-25 Published:2014-04-25
  • Contact: Xiaojian Cao, Ph.D., Department of Orthopedics, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China, xiaojiancao@gmail.com.
  • Supported by:

    This work was supported by the National Natural Science Foundation of China, No.  81171694, 81201374, 81371968, 81371969; a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions; and the Nature Science Foundation of Jiangsu Province No. BK2012718, BK2011844.

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摘要：

在局部应用丝裂霉素C有效抑制椎板切除后脊髓硬膜外瘢痕粘连时，有研究显示与浓度有关可对视神经与听神经产生一定的神经毒性。为验证安全的丝裂霉素C的浓度，实验在切除大鼠L1椎板后，使用浸有0.1，0.3，0.5，0.7 mg/mL的丝裂霉素C的棉片在损伤区局部湿敷5min，以大鼠坐骨神经替代背神经进行研究。结果显示，0.1-0.5mg/mL的丝裂霉素C对大鼠坐骨神经的结构和功能无明显影响；0.7 mg/mL的丝裂霉素C虽未影响其功能，但能明显减少坐骨神经髓鞘厚度。说明局部应用低浓度丝裂霉素C治疗椎板切除后瘢痕粘连是安全的，但高浓度丝裂霉素C（大于0.7 mg/mL）对周围神经功能存在潜在危险。

关键词: 神经再生, 周围神经损伤, 丝裂霉素C, 髓鞘, 椎板切除, 电生理, 神经功能, NSFC grant

Abstract:

Although the local application of mitomycin C may prevent epidural adhesion after laminectomy, mitomycin C can induce neurotoxicity in optic and acoustic nerves at high concentrations. To determine the safe concentration range for mitomycin C, cotton pads soaked with mitomycin C at different concentrations (0.1, 0.3, 0.5, and 0.7 mg/mL) were immediately applied for 5 minutes to the operation area of rats that had undergone laminectomy at L1. Rat sciatic nerves, instead of dorsal nerves, were used in this study. The results showed that mitomycin C at 0.1–0.5 mg/mL did not damage the structure and function of the sciatic nerve, while at 0.7 mg/mL, mitomycin C significantly reduced the thickness of the sciatic nerve myelin sheath compared with lower concentrations, though no functional change was found. These experimental findings indicate that the local application of mitomycin C at low concentrations is safe to prevent scar adhesion following laminectomy, but that mitomycin C at high concentrations (> 0.7 mg/mL) has potential safety risks to peripheral nerve structures.

Key words: nerve regeneration, peripheral nerve injury, mitomycin C, myelin sheath, laminectomy, electrophysiology, nerve function,  , NSFC grant, neural regeneration