中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (10): 1014-1019.doi: 10.4103/1673-5374.133160

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

双层不等隙胶原膜支架负载神经干细胞移植修复损伤脊髓

  

  • 收稿日期:2014-04-11 出版日期:2014-05-26 发布日期:2014-05-26

Neural stem cell transplantation in a double-layer collagen membrane with unequal pore sizes for spinal cord injury repair

Ning Yuan 1, Wei Tian 1, Lei Sun 2, Runying Yuan 2, Jianfeng Tao 2, Dafu Chen 2   

  1. 1 Department of Spine, Beijing Jishuitan Hospital, Beijing, China
    2 Beijing Institute of Orthopedics and Traumatology, Beijing, China
  • Received:2014-04-11 Online:2014-05-26 Published:2014-05-26
  • Contact: Wei Tian, M.D., Department of Spine, Beijing Jishuitan Hospital, Beijing 100035, China, tianweijst@vip.163.com.

摘要:

实验设计了一种具有双层结构的新型神经干细胞支架双层不等隙胶原膜,内层为孔隙较小的疏松层,外层为孔隙较大的致密层。在修复大鼠不完全脊髓损伤时,将大量神经干细胞浸润于双层不等隙胶原膜多孔隙的疏松层,将疏松层贴于损伤侧,而双层不等隙胶原膜致密层置于外侧。结果显示双层不等隙胶原膜结合神经干细胞移植治疗的脊髓不完全损伤模型大鼠脊髓损伤部位神经干细胞出现分化,病理损害明显减轻,且大鼠的运动功能明显增强。说明双层不等隙胶原膜支架负载神经干细胞移植可以有效修复损伤脊髓。

关键词: 神经再生, 脊髓损伤, 胶原, 支架, 神经干细胞, 细胞移植, 神经修复

Abstract:

A novel double-layer collagen membrane with unequal pore sizes in each layer was designed and tested in this study. The inner, loose layer has about 100-μm-diameter pores, while the outer, compact layer has about 10-μm-diameter pores. In a rat model of incomplete spinal cord injury, a large number of neural stem cells were seeded into the loose layer, which was then adhered to the injured side, and the compact layer was placed against the lateral side. The results showed that the transplantation of neural stem cells in a double-layer collagen membrane with unequal pore sizes promoted the differentiation of neural stem cells, attenuated the pathological lesion, and significantly improved the motor function of the rats with incomplete spinal cord injuries. These experimental findings suggest that the transplantation of neural stem cells in a double-layer collagen membrane with unequal pore sizes is an effective therapeutic strategy to repair an injured spinal cord.

Key words: nerve regeneration, spinal cord injury, collagen, scaffolds, neural stem cells, cell transplantation, nerve repair, neural regeneration