中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (17): 1616-1620.doi: 10.4103/1673-5374.141789

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

质子磁共振波谱不能发现氯化甲基汞对成年大鼠海马结构的损伤

  

  • 收稿日期:2014-06-18 出版日期:2014-09-16 发布日期:2014-09-16
  • 基金资助:

    中国国家自然科学基金项目 (81060231,81160338);湖北省自然科学基金项目(2013CFB277)

Methylmercury chloride damage to the adult rat hippocampus cannot be detected by proton magnetic resonance spectroscopy

Zhiyan Lu 1, Jinwei Wu 1, 2, Guangyuan Cheng 1, Jianying Tian 3, Zeqing Lu 4, Yongyi Bi 5   

  1. 1 Department of Radiology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei Province, China
    2 Department of Radiology, Hainan Provincial Nongken Hospital, Haikou, Hainan Province, China
    3 Department of Anatomy, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, China
    4 School of Public Health, University of Minnesota, Minnesota, MN, USA
    5 School of Public Health, Wuhan University, Wuhan, Hubei Province, China
  • Received:2014-06-18 Online:2014-09-16 Published:2014-09-16
  • Contact: Yongyi Bi, Ph.D., School of Public Health, Wuhan University, Wuhan 430071, Hubei Province, China, yongyib@aliyun.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81060231, 81160338; Hubei Province Natural Science Foundation of China, No. 2013CFB277.

摘要:

既往研究发现甲基汞能破坏海马神经元从而引起认知功能障碍,但到目前为止,还未见无创、安全、精确的大脑海马损伤的检测方法。实验拟探索能否运用质子磁共振波谱检测甲基汞对脑海马组织产生的损害。给予大鼠颈部皮下连续50d注射4,2mg/kg甲基汞,经水迷宫及病理学检测证实其认知功能受损,海马组织超微结构遭到破坏,但质子磁共振波谱结果却显示大鼠大脑海马组织中氮-乙酰天门冬氨酸/肌酸、胆碱类复合物/肌酸及肌醇/肌酸比值未出现明显变化。提示质子磁共振波谱不能用于检测氯化甲基汞对成年大鼠海马结构损伤的影响。

关键词: 神经再生, 质子磁共振波谱, 氯化甲基汞, 认知功能障碍, 海马, 行为学, 病理学, NSFC grant

Abstract:

Previous studies have found that methylmercury can damage hippocampal neurons and accordingly cause cognitive dysfunction. However, a non-invasive, safe and accurate detection method for detecting hippocampal injury has yet to be developed. This study aimed to detect methylmercury-induced damage on hippocampal tissue using proton magnetic resonance spectroscopy. Rats were given a subcutaneous injection of 4 and 2 mg/kg methylmercury into the neck for 50 consecutive days. Water maze and pathology tests confirmed that cognitive function had been impaired and that the ultrastructure of hippocampal tissue was altered after injection. The results of proton magnetic resonance spectroscopy revealed that the nitrogen-acetyl aspartate/creatine, choline complex/creatine and myoinositol/creatine ratio in rat hippocampal tissue were unchanged. Therefore, proton magnetic resonance spectroscopy can not be used to determine structural damage in the adult rat hippocampus caused by methylmercury chloride.

Key words: nerve regeneration, proton magnetic resonance spectroscopy, methylmercury chloride, cognitive dysfunction, hippocampus, behavior, pathology, NSFC grant, neural regeneration