中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (21): 1907-1911.doi: 10.4103/1673-5374.143435

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

成年四线草鼠大脑的神经发生现象

  

  • 收稿日期:2014-07-04 出版日期:2014-11-15 发布日期:2014-11-15

Adult neurogenesis in the four-striped mice (Rhabdomys pumilio)

Olatunbosun O. Olaleye 1, 2, Amadi O. Ihunwo 2   

  1. 1 Department of Oral Biological Sciences, School of Oral Health Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
    2 School of Anatomical Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
  • Received:2014-07-04 Online:2014-11-15 Published:2014-11-15
  • Contact: Amadi O. Ihunwo, School of Anatomical Sciences, Faculty of Health Sciences, University of the Witwatersrand, 7 York Road, Parktown, 2193, Johannesburg, South Africa, Amadi.Ihunwo@wits.ac.za.
  • Supported by:

    This project was supported by Individual Faculty Research Grant and Swiss-South Africa Joint Research Progamme (SSAJRP).

摘要:

为研究自然环境下四线草鼠成年大鼠神经发生情况,应用免疫组化方法检测大鼠大脑增殖细胞标志物Ki-67和未成熟神经元标志物DCX的免疫反应。结果显示,大鼠侧脑室脑室下区活性带,直至嗅球、海马齿状回的颗粒下层检测到Ki-67和DCX免疫阳性细胞,在纹状体、第三脑室、大脑皮质、杏仁体、嗅球、沿吻侧迁移流也检测到DCX免疫阳性细胞。说明四线草鼠成年后大脑仍能不断产生新的神经元,存在神经发生现象。
 

关键词: 成体神经发生, 四线草鼠, Ki-67, DCX, 脑室下区, 齿状回, 嗅球, 神经可塑性

Abstract:

In this study, we investigated non-captive four-striped mice (Rhabdomys pumilio) for evidence that adult neurogenesis occurs in the adult brain of animal models in natural environment. Ki-67 (a marker for cell proliferation) and doublecortin (a marker for immature neurons) immunostaining confirmed that adult neurogenesis occurs in the active sites of subventricular zone of the lateral ventricle with the migratory stream to the olfactory bulb, and the subgranular zone of the dentate gyrus of the hippocampus. No Ki-67 proliferating cells were observed in the striatum substantia nigra, amygdala, cerebral cortex or dorsal vagal complex. Doublecortin-immunoreactive cells were observed in the striatum, third ventricle, cerebral cortex, amygdala, olfactory bulb and along the rostral migratory stream but absent in the substantia nigra and dorsal vagal complex. The potential neurogenic sites in the four-striped mouse species could invariably lead to increased neural plasticity.

Key words: nerve regeneration, adult neurogenesis, four-striped mouse, Ki-67, doublecortin, subventricular zone, dentate gyrus, olfactory bulb, potential neurogenic site, neural plasticity, neural regeneration