中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (23): 2066-2073.doi: 10.4103/1673-5374.147933

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

黄芩茎叶总黄酮预处理对脑缺血再灌注损伤大脑海马神经元的预防性保护

  

  • 收稿日期:2014-08-19 出版日期:2014-12-10 发布日期:2014-12-10

Pretreatment with scutellaria baicalensis stem-leaf total flavonoid protects against cerebral ischemia/reperfusion injury in hippocampal neurons

Xiangyu Kong, Wei Kong, Guangxin Miao, Shumin Zhao, Meng Chen, Xiaoying Zheng, Jiangtao Bai   

  1. Laboratory of Spinal Cord Injury and Rehabilitation, Chengde Medical College, Chengde, Hebei Province, China
  • Received:2014-08-19 Online:2014-12-10 Published:2014-12-10
  • Contact: Shumin Zhao, Laboratory of Spinal Cord Injury and Rehabilitation, Chengde Medical College, Chengde 067000, Hebei Province, China, zhaoshumin-2008@163.com.
  • Supported by:

    This study was supported by the grants from Hebei Provincial Science and Technology  Department, No. 07276101D-46.

摘要:

以往实验研究表明黄芩茎叶总黄酮对脑梗死治疗有效,但在用于预防性保护方面报道较少。实验以50,100,200mg/kg黄芩茎叶总黄酮连续灌胃大鼠1周后建立局灶性大脑缺血再灌注模型。结果显示,黄芩茎叶总黄酮预处理能有效改善脑缺血再灌注大鼠神经功能,降低脑组织含水量及脑血管通透性,改善海马微血管形态,降低海马神经元中海马神经元中Fas和FasL的表达,提升脑组织中超氧化物歧化酶和谷胱甘肽过氧化物酶活力,降低丙二醛含量,且中、高浓度黄芩茎叶总黄酮的作用效果较低浓度更为显著。提示黄芩茎叶总黄酮预处理对大脑脑缺血再灌注损伤具有预防性保护作用,其作用机制是降低缺血再灌注脑含水量,增加微血管再通,抑制海马神经元凋亡,减轻自由基损伤的多途径、多环节、多靶点联合作战的结果。

关键词: 神经再生, 黄芩茎叶总黄酮, 预处理, 脑缺血再灌注, 海马, 凋亡, 脑血管通透性, 自由基

Abstract:

Previous experimental studies have shown that cerebral infarction can be effectively reduced following treatment with scutellaria baicalensis stem-leaf total flavonoid (SSTF). However, the mechanism of action of SSTF as a preventive drug to treat cerebral infarction remains unclear. In this study, Sprague-Dawley rats were pretreated with 50, 100, 200 mg/kg SSTF via intragastric administration for 1 week prior to the establishment of focal cerebral ischemia/reperfusion injury. The results showed that pretreatment with SSTF effectively improved neurological function, reduced brain water content and the permeability of blood vessels, ameliorated ischemia-induced morphology changes in hippocampal microvessels, down-regulated Fas and FasL protein expression, elevated the activity of superoxide dismutase and glutathione peroxidase, and decreased malondialdehyde content. In contrast to low-dose SSTF pretreatment, the above changes were most obvious after pretreatment with moderate- and high-doses of SSTF. Experimental findings indicate that SSTF pretreatment can exert protective effects on the brain against cerebral ischemia/reperfusion injury. The underlying mechanisms may involve reducing brain water content, increasing microvascular recanalization, inhibiting the apoptosis of hippocampal neurons, and attenuating free radical damage.

Key words: nerve regeneration, scutellaria baicalensis stem-leaf total flavonoid, pretreatment, cerebral ischemia/reperfusion, hippocampus, apoptosis, vascular permeability, free radicals, neural regeneration