中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (24): 2205-2210.doi: 10.4103/1673-5374.147954

• 原著:脊髓损伤修复保护与再生 • 上一篇    

褪黑素可减轻脊髓损伤后的水肿

  

  • 收稿日期:2014-11-23 出版日期:2014-12-25 发布日期:2014-12-25

Melatonin lowers edema after spinal cord injury

Cheng Li 1, Xiao Chen 1, Suchi Qiao 1, Xinwei Liu 2, Chang Liu 1, Degang Zhu 1, Jiacan Su 1, Zhiwei Wang 1   

  1. 1 Department of Orthopedic Surgery, Changhai Hospital, the Second Military Medical University, Shanghai, China
    2 Laboratory of Severe and War-Related Trauma Center, General Hospital of Shenyang Military Area Command of Chinese PLA, Shenyang,
    Liaoning Province, China
  • Received:2014-11-23 Online:2014-12-25 Published:2014-12-25
  • Contact: Zhiwei Wang, M.D., Department of Orthopedic Surgery, Changhai Hospital, the Second Military Medical University, Shanghai 200433, China, wangzhiweils@126.com.
  • Supported by:

    This study was supported by the China Postdoctoral Science Foundation Grant, No. 2014M552692.

摘要:

一些研究者通过动物实验发现褪黑素具有抗水肿作用,可用于脊髓损伤的治疗。为此,实验提出了以褪黑素减轻损伤脊髓水肿的假设。经实验验证后发现,100 mg/kg褪黑素腹腔注射可减少钳夹伤脊髓含水量,并抑制损伤脊髓水通道蛋白4和胶质纤维酸性蛋白的表达。说明褪黑素能有效减轻损伤脊髓的水肿,其作用机制与抑制水通道蛋白4和胶质纤维酸性蛋白的表达有关。

关键词: 神经再生, 脊髓损伤, 褪黑素, 水肿, 水通道蛋白, 胶质纤维酸性蛋白, 免疫组织化学染色, 苏木精-伊红染色, 免疫印迹

Abstract:

Melatonin has been shown to diminish edema in rats. Melatonin can be used to treat spinal cord injury. This study presumed that melatonin could relieve spinal cord edema and examined how it might act. Our experiments found that melatonin (100 mg/kg, i.p.) could reduce the water content of the spinal cord, and suppress the expression of aquaporin-4 and glial fibrillary acidic protein after spinal cord injury. This suggests that the mechanism by which melatonin alleviates the damage to the spinal cord by edema might be related to the expression of aquaporin-4 and glial fibrillary acidic protein.

Key words: nerve regeneration, spinal cord injury, melatonin, edema, aquaporin, glial fibrillary acidic protein, immunohistochemistry, western blot assay, neural regeneration