中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (4): 610-617.doi: 10.4103/1673-5374.155436

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

白细胞介素6以时间和剂量依赖方式调控大脑皮质神经元电压门控Na+通道发挥神经保护效应

  

  • 收稿日期:2015-02-07 出版日期:2015-04-22 发布日期:2015-04-22
  • 基金资助:

    国家自然科学基金(30972766, 31170852, 81001322, 81172795, 81173048)以及高等学校博士学科点专项科研基金 (20094402110004) 资助。

Neuroprotective effect of interleukin-6 regulation of voltage-gated Na+ channels of cortical neurons is time- and dose-dependent

Wei Xia 1, Guo-yi Peng 2, Jiang-tao Sheng 3, Fang-fang Zhu 3, Jing-fang Guo 2, Wei-qiang Chen 2   

  1. 1 Department of Interventional Radiology, First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong Province, China
    2 Department of Neurosurgery, First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong Province, China
    3 Department of Pathogenic Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong Province, China
  • Received:2015-02-07 Online:2015-04-22 Published:2015-04-22
  • Contact: Wei-qiang Chen, M.D., Ph.D., 2274536975@qq.com.
  • Supported by:

     This work was supported by a grant from the National Natural Science Foundation of China, No. 30972766, 31170852, 81001322, 81172795, 81173048; and the Specialized Research Fund for the Doctoral Program of Colleges and Universities, No. 20094402110004.

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摘要：

尽管有足够的证据表明白细胞介素6既参与了神经损伤性过程又与神经再生等保护性的过程相关，但长期高浓度白细胞介素6干预对中枢神经系统神经元的影响仍鲜为人知。因此，我们利用膜片钳技术来探索不同浓度(0.1，1，5，10 ng/mL)白细胞介素6作用大脑皮质神经元24 h以及不同干预时长(10 ng/mL白细胞介素6处理皮质神经元2，4，8，24，48 h)对电压门控Na+通道的影响。电压钳结果表明，白细胞介素6通过其受体以时间和剂量依赖的方式抑制皮质神经元Na+电流，但不改变其激活和失活性质。电流钳记录结果与电压钳记录结果相一致，白细胞介素6降低皮质神经元动作电位幅度但不影响动作电位阈值的变化。说明白细胞介素6以时间和剂量依赖的方式调控大鼠皮质神经元电压门控Na+通道。

关键词: 神经再生, 脑损伤, 炎症反应, 白细胞介素6, 电压门控钠通道, 皮质神经元, 脑脊液, 神经免疫, 神经保护, 动作电位, 膜片钳, 神经电生理, 国家自然科学基金

Abstract:

Interleukin-6 has been shown to be involved in nerve injury and nerve regeneration, but the effects of long-term administration of high concentrations of interleukin-6 on neurons in the central nervous system is poorly understood. This study investigated the effects of 24 hour exposure of interleukin-6 on cortical neurons at various concentrations (0.1, 1, 5 and 10 ng/mL) and the effects of 10 ng/mL interleukin-6 exposure to cortical neurons for various durations (2, 4, 8, 24 and 48 hours) by studying voltage-gated Na+ channels using a patch-clamp technique. Voltage-clamp recording results demonstrated that interleukin-6 suppressed Na+ currents through its receptor in a time- and dose-dependent manner, but did not alter voltage-dependent activation and inactivation. Current-clamp recording results were consistent with voltage-clamp recording results. Interleukin-6 reduced the action potential amplitude of cortical neurons, but did not change the action potential threshold. The regulation of voltage-gated Na+ channels in rat cortical neurons by interleukin-6 is time- and dose-dependent.

Key words: nerve regeneration, brain injury, inflammatory reaction, interleukin-6, voltage-gated Na+ channel, cortical neurons, cerebrospinal fluid, neuroimmunomodulation, neuroprotection,  , action potential, patch clamp, neurophysiology, NSFC grants, neural regeneration