中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (5): 766-771.doi: 10.4103/1673-5374.157243

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

侧脑室注射Apelin-13抑制脑缺血再灌注损伤后的细胞凋亡

  

  • 收稿日期:2015-02-12 出版日期:2015-05-15 发布日期:2015-05-15
  • 基金资助:

    国家自然科学基金 (30971081, 31271243, 81070961 and 81241052), 山东省自然科学基金 (ZR2011CM027, 2012GGA08100).

Lateral intracerebroventricular injection of Apelin-13 inhibits apoptosis after cerebral ischemia/reperfusion injury

Xiao-ge Yan 1, Bao-hua Cheng 2, Xin Wang 2, Liang-cai Ding 2, Hai-qing Liu 3, Jing Chen 2, Bo Bai 2   

  1. 1 Xinxiang Medical University, Xinxiang, Henan Province, China
    2 Neurobiology Institute, Jining Medical University, Jining, Shandong Province, China
    3 Taishan Medical University, Taian, Shandong Province, China
  • Received:2015-02-12 Online:2015-05-15 Published:2015-05-15
  • Contact: Bo Bai, M.D., bbai@mail.jnmc.edu.cn
  • Supported by:

    This study was financially supported by the National Natural Science Foundation of China, No. 30971081, 31271243, 81070961 and 81241052; and the Natural Science Foundation of Shandong Province of China, No. ZR2011CM027 and 2012GGA08100.

摘要:

Apelin-13可抑制过氧化氢诱导的神经细胞凋亡,但其对脑缺血再灌注损伤中神经细胞凋亡的影响研究尚未见报道。为此,实验给予MCAO模型大鼠进行侧脑室注射Apelin-13(0.1 μg/g)。TTC染色,TUNEL染色,免疫组织化学染色检测显示,与脑缺血再灌注组相比,Apelin-13干预组大鼠脑梗死体积减少,脑缺血半暗带凋亡细胞数量减少,Bcl-2表达上调,Caspase-3阳性细胞数量减少,说明Apelin-13可以通过抑制大鼠脑缺血再灌注损伤的神经细胞凋亡来发挥神经保护作用。

关键词: 神经再生, 脑损伤, Apelin-13, 神经保护, 侧脑室注射, 脑缺血-再灌注损伤, 神经细胞凋亡, MCAO, TTC, TUNEL, Bcl-2, Caspase-3, 国家自然科学基金

Abstract:

Apelin-13 inhibits neuronal apoptosis caused by hydrogen peroxide, yet apoptosis following cerebral ischemia-reperfusion injury has rarely been studied. In this study, Apelin-13 (0.1 μg/g) was injected into the lateral ventricle of middle cerebral artery occlusion model rats. TTC, TUNEL, and immunohistochemical staining showed that compared with the cerebral ischemia/reperfusion group, infarct volume and apoptotic cell number at the ischemic penumbra region were decreased in the Apelin-13 treatment group. Additionally, Apelin-13 treatment increased Bcl-2 immunoreactivity and decreased caspase-3 immunoreactivity. Our findings suggest that Apelin-13 is neuroprotective against cerebral ischemia/reperfusion injury through inhibition of neuronal apoptosis.

Key words: nerve regeneration, brain injury, neuroprotection, cerebral ischemia/reperfusion injury, lateral intracerebroventricular injection, Apelin-13, nerve apoptosis, Bcl-2, caspase-3, NSFC grants, neural regeneration