中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (6): 944-950.doi: 10.4103/1673-5374.158359
幼年短暂性全脑缺血海马神经元延迟死亡与p63表达相关
Delayed hippocampal neuronal death in young gerbil following transient global cerebral ischemia is related to higher and longer-term expression of p63 in the ischemic hippocampus
Eun Joo Bae 1, Bai Hui Chen 2, Bing Chun Yan 3, Bich Na Shin 2, Jeong Hwi Cho 4, In Hye Kim 4, Ji Hyeon Ahn 4, Jae Chul Lee 4, Hyun-Jin Tae 5, Seongkweon Hong 6, Dong Won Kim 7, 8, Jun Hwi Cho 8, Yun Lyul Lee 2, Moo-Ho Won 4, Joon Ha Park 4
- 1 Department of Pediatrics, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, Chuncheon, South Korea
2 Department of Physiology, College of Medicine, Institute of Neurodegeneration and Neuroregeneration, Hallym University, Chuncheon, South Korea
3 Institute of Integrative Traditional & Western Medicine, Medical College, Yangzhou University, Yangzhou, Jiangsu Province, China
4 Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, South Korea
5 Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon, South Korea
6 Department of Surgery, School of Medicine, Kangwon National University, Chuncheon, South Korea
7 Department of Emergency Medicine, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, Chuncheon, South Korea
8 Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon, South Korea
摘要：
p53在脑缺血大脑中的变化已有报道，但其家族成员肿瘤抑制因子p63在全脑缺血海马中的表达变化研究报道较少。为此，实验比较了幼年（鼠龄1个月）和成年（鼠龄6个月）经历5min短暂性全脑缺血后海马CA1-3区p63的表达。发现幼年沙鼠海马CA1区神经元死亡的发生较成年沙鼠延迟。成年沙鼠海马CA1区锥体神经元p63免疫反应在缺血后4d明显降低，且明显低于幼年沙鼠。缺血后7d，成年和幼年沙鼠海马CA1区锥体神经元p63免疫反应均减弱，p63蛋白表达在幼年和成年沙鼠海马CA1区随缺血时间的变化与其免疫反应相似。说明幼年相较于成年海马CA1区神经元死亡延迟的原因，与p63表达在较长时间内维持较高水平有关。