中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2020, Vol. 15 ›› Issue (3): 548-556.doi: 10.4103/1673-5374.266063

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

油莎草活性单体荭草苷对脑缺血再灌注损伤的保护

  

  • 出版日期:2020-03-15 发布日期:2020-05-26
  • 基金资助:
    国家自然科学基金（31770385）

Neuroprotection of Cyperus esculentus L. orientin against cerebral ischemia/reperfusion induced brain injury

Si-Qun Jing1, Sai-Sai Wang2, Rui-Min Zhong1, Jun-Yan Zhang1, Jin-Zi Wu3, Yi-Xian Tu4, Yan Pu4, Liang-Jun Yan3   

  1. 1 Yingdong College of Food Science and Engineering, Shaoguan University, Shaoguan, Guangdong Province, China
    2 School of Bioengineering, Jiangnan University, Wuxi, Jiangsu Province, China
    3 Department of Pharmaceutical Sciences, UNT System College of Pharmacy, University of North Texas Health Science Center, Fort Worth, TX, USA
    4 College of Life Sciences and Technology, Xinjiang University, Urumqi, Xinjiang Uygur Autonomous Region, China
  • Online:2020-03-15 Published:2020-05-26
  • Contact: Si-Qun Jing, PhD,jingsiqun@163.com; Liang-Jun Yan, PhD,liang-jun.yan@unthsc.edu.
  • Supported by:
    This work was supported by the National Natural Science Foundation of China, No. 31770385 (to SQJ).

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摘要：

荭草苷是一种类黄酮单体化合物，具有抗心肌缺血、抗凋亡、抗辐射、抗肿瘤和抗衰老等生物活性。然而，关于荭草苷对脑卒中损伤的神经保护作用尚未得到全面研究。因此，实验以荭草苷标品(STO)作对照，观察油莎草活性单体荭草苷(CLO)对脑缺血/再灌注(I/R)损伤的神经保护作用及机制。首先进行体外实验，采用CoCl2诱导鼠海马神经细胞系HT22细胞，建立缺血/再灌注损伤细胞模型后用CLO干预，对照组细胞正常培养；体内实验采用线栓法建立大脑中动脉闭塞(MCAO)大鼠模型，采用CLO灌胃(125，500 mg/kg)。(1)体外实验的氧化应激能力检测及Western blot检测显示，与对照组比较，CLO可通过降低脂质过氧化水平和活性氧含量，抑制Caspase-3蛋白表达，从而发挥CLO对CoCl2诱导的HT22细胞缺血性损伤的保护作用，但CLO未减少乳酸脱氢酶含量的释放，也未增加超氧化物歧化酶的活性；(2)体内实验结果显示，CLO可降低MCAO大鼠神经功能缺损评分，减少脑组织含水量和脑梗塞体积，从而在脑缺血再灌注损伤中发挥神经保护作用；(3)以上研究结果表明，CLO对脑缺血再灌注致脑损伤的保护作用机制可能与其抗氧化能力及抑制Caspase-3蛋白的激活有关。实验方案已于2016-05-16在中国新疆医科大学实验动物伦理委员会批准，批准号：IACUC20160516-57。

orcid: 0000-0002-2937-3594 (Si-Qun Jing) 

         0000-0002-5815-5430 (Liang-Jun Yan)

关键词: 油莎草活性单体荭草苷(CLO), 抗氧化性, 脑缺血再灌注损伤, Caspase-3, CoCl2, 神经功能缺损, 氧化应激, 活性氧, 脂质过氧化

Abstract: Orientin is a flavonoid monomer. In recent years, its importance as a source of pharmacological active substance is growing rapidly due to its properties such as anti-myocardial ischemia, anti-apoptosis, anti-radiation, anti-tumor, and anti-aging. However, the neuroprotective effects of Orientin on stroke injury have not been comprehensively evaluated. The aim of the present study was thus to investigate the neuroprotective capacity and the potential mechanisms of Cyperus esculentus L. orientin (CLO) from Cyperus esculentus L. leaves against ischemia/ reperfusion (I/R) injury using standard orientin as control. For in vitro studies, we treated HT22 cells with CoCl2 as an in vitro ischemic injury model. HT22 cells in the control group were treated with CoCl2. For in vivo studies, we used rat models of middle cerebral artery occlusion, and animals that received sham surgery were used as controls. We found that CLO protected CoCl2-induced HT22 cells against ischemia/reperfusion injury by lowering lipid peroxidation and reactive oxygen species formation as well as decreasing protein oxidation. However, CLO did not reduce the release of lactate dehydrogenase nor increase the activity of superoxide dismutase. Results showed that CLO could decrease neurological deficit score, attenuate brain water content, and reduce cerebral infarct volume, leading to neuroprotection during cerebral ischemia-reperfusion injury. Our studies indicate that CLO flavonoids can be taken as a natural antioxidant and bacteriostastic substance in food and pharmaceutical industry. The molecular mechanisms of CLO could be at least partially attributed to the antioxidant properties and subsequently inhibiting activation of casepase-3. All experimental procedures and protocols were approved on May 16, 2016 by the Experimental Animal Ethics Committee of Xinjiang Medical University of China (approval No. IACUC20160516-57).

Key words: antioxidants, caspase-3, cerebral ischemia/reperfusion injury, cobalt chloride, Cyperus esculentus L. orientin (CLO), lipid peroxidation, nerve regeneration, neurological deficits, oxidative stress, reactive oxygen species