关键词: 神经再生, 脑损伤, 癫痫, 神经保护, 脑内炎症, 凋亡, 核因子κB, 白细胞介素6, 白细胞介素1β, 诱导型一氧化氮合酶, 一氧化氮, 前列腺素E2, 国家自然科学基金

Abstract:

Amentoflavone is a natural biflavone compound with many biological properties, including anti-inflammatory, antioxidative, and neuroprotective effects. We presumed that amentoflavone exerts a neuroprotective effect in epilepsy models. Prior to model establishment, mice were intragastrically administered 25 mg/kg amentoflavone for 3 consecutive days. Amentoflavone effectively prevented pilocarpine-induced epilepsy in a mouse kindling model, suppressed nuclear factor-κB activation and expression, inhibited excessive discharge of hippocampal neurons resulting in a reduction in epileptic seizures, shortened attack time, and diminished loss and apoptosis of hippocampal neurons. Results suggested that amentoflavone protected hippocampal neurons in epilepsy mice via anti-inflammation, antioxidation, and antiapoptosis, and then effectively prevented the occurrence of seizures.

Key words: nerve regeneration, brain injury, epilepsy, neuroprotection, apoptosis, nuclear factor-κB, brain inflammation, interleukin-6, interleukin-1 beta, inducible nitric oxide synthase, nitric oxide, prostaglandin E2, NSFC grant, neural regeneration