中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (8): 1245-1250.doi: 10.4103/1673-5374.162756

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

运动训练预处理可发挥对脑缺血后海马CA1区神经元损害的保护效应

  

  • 收稿日期:2015-07-04 出版日期:2015-08-24 发布日期:2015-08-24
  • 基金资助:

    伊朗国家科学基金(91052159)

Exercise preconditioning exhibits neuroprotective effects on hippocampal CA1 neuronal damage after cerebral ischemia

Nabi Shamsaei1, Mehdi Khaksari2, Sohaila Erfani3, Hamid Rajabi4, Nahid Aboutaleb5, *   

  1. 1 Department of Physical Education & Sports Science, Faculty of Literature and Humanities, Ilam University, Ilam, Iran
    2 School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
    3 Department of Animal Physiology, Faculty of Biology, Kharazmi University, Tehran, Iran
    4 Faculty of Physical Education & Sports Science, Kharazmi University, Tehran, Iran
    5 Physiology Research Center and Department of Physiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Received:2015-07-04 Online:2015-08-24 Published:2015-08-24
  • Contact: Nahid Aboutaleb, M.D., aboutalebn8@gmail.com.
  • Supported by:

    This study was supported by a grant (under the contract number 91052159) sponsored by the Iran National Science Foundation (INSF).

摘要:

最近研究显示,运动训练对脑缺血有神经保护作用,但其对脑缺血致海马损害的作用尚存在争议。为研究脑缺血前进行平板运动训练对海马CA1区神经元损害的影响。实验将成年雄性大鼠随机分为对照组、缺血和运动+缺血组。运动+缺血组大鼠在建立脑缺血模型前给予4周的平板运动训练。缺血后4d,以被动避暗试验评估大鼠学习记忆功能,以尼氏染色和TUNEL染色检测海马神经元损害情况。发现运动预处理明显减轻海马CA1区神经元损害程度,减少凋亡细胞数量,改善脑缺血大鼠学习记忆功能。说明运动训练预处理可通过挽救缺血诱导的海马CA1区神经元退变,改善脑缺血大鼠学习记忆功能。

关键词: 神经再生, 运动训练, 脑缺血, 海马, 凋亡, TUNEL, 尼氏染色, 记忆

Abstract:

Recent evidence has suggested the neuroprotective effects of physical exercise on cerebral ischemic injury. However, the role of physical exercise in cerebral ischemia-induced hippocampal damage remains controversial. The aim of the present study was to evaluate the effects of pre-ischemia treadmill training on hippocampal CA1 neuronal damage after cerebral ischemia. Male adult rats were randomly divided into control, ischemia and exercise + ischemia groups. In the exercise + ischemia group, rats were subjected to running on a treadmill in a designated time schedule (5 days per week for 4 weeks). Then rats underwent cerebral ischemia induction through occlusion of common carotids followed by reperfusion. At 4 days after cerebral ischemia, rat learning and memory abilities were evaluated using passive avoidance memory test and rat hippocampal neuronal damage was detected using Nissl and TUNEL staining. Pre-ischemic exercise significantly reduced the number of TUNEL-positive cells and necrotic cell death in the hippocampal CA1 region as compared to the ischemia group. Moreover, pre-ischemic exercise significantly prevented ischemia-induced memory dysfunction. Pre-ischemic exercise mighct prevent memory deficits after cerebral ischemia through rescuing hippocampal CA1 neurons from ischemia-induced degeneration.

Key words: nerve regeneration, physical exercise, cerebral ischemia, hippocampus, apoptosis, Nissl staining, TUNEL, memory, neural regeneration