中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (8): 1321-1323.doi: 10.4103/1673-5374.162767

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

Notch1基因表达变化揭示补阳还五汤保护损伤脊髓的干预靶点

  

  • 收稿日期:2015-06-19 出版日期:2015-08-24 发布日期:2015-08-24
  • 基金资助:

    辽宁省大学生创新项目(201310160016)

Buyang Huanwu decoction up-regulates Notch1 gene expression in injured spinal cord

Zhan-peng Guo1, Mi-na Huang2, An-qi Liu3, Ya-jiang Yuan1, Jian-bo Zhao1, Xi-fan Mei1   

  1. 1 Institute of Orthopedics, the First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning Province, China
    2 College of Nursing, Liaoning Medical University, Jinzhou, Liaoning Province, China
    3 College of Basic Medical Sciences, Liaoning Medical University, Jinzhou, Liaoning Province, China
  • Received:2015-06-19 Online:2015-08-24 Published:2015-08-24
  • Contact: Xi-fan Mei, M.D., 153587486@qq.com.
  • Supported by:

    This study was supported by a grant from the University Students’ Innovation and Entrepreneurship Training Program in Liaoning Province of China, No. 201310160016.

摘要:

作者既往研究发现,受损脊髓组织中Notch信号通路的基因表达出现明显变化,说明其参与脊髓损伤过程。补阳还五汤具有促进再生神经纤维生长的作用,是否可影响损伤脊髓Notch信号通路尚不可知。实验以打击法建立T10脊髓钝性击打伤模型大鼠,损伤后第2天起每天以2 mL补阳还五汤(0.8g/mL)灌胃干预至进行检测。实时反转录PCR检测发现,损伤后7,14,28d后,补阳还五汤干预大鼠损伤脊髓组织中Notch1基因表达明显上升。说明补阳还五汤促进损伤脊髓中Notch1基因的表达,从而揭示了补阳还五汤发挥神经保护作用的干预靶点。

关键词: 神经再生, 补阳还五汤, 脊髓损伤, Notch1, Notch1信号通路, 中药

Abstract:

Expression of genes in the Notch signaling pathway is altered in the injured spinal cord, which indicates that Notch participates in repair after spinal cord injury. Buyang Huanwu decoction, a traditional Chinese herbal preparation, can promote the growth of nerve cells and nerve fibers; however, it is unclear whether Buyang Huanwu decoction affects the Notch signaling pathway in injured spinal cord. In this study, a rat model was established by injuring the T10 spinal cord. At 2 days after injury, rats were intragastrically administered 2 mL of 0.8 g/mL Buyang Huanwu decoction daily until sacrifice. Real-time reverse transcription polymerase chain reaction analysis demonstrated that at 7, 14 and 28 days after injury, the expression of Notch1 was increased in the Buyang Huanwu decoction group compared with controls. These findings confirm that Buyang Huanwu decoction can promote the expression of Notch1 in rats with incomplete spinal cord injury, and may indicate a mechanism to promote the repair of spinal cord injury.

Key words: nerve regeneration, Buyang Huanwu decoction, spinal cord injury, Notch1 signaling pathway, Chinese medicine, neural regeneration