中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (10): 1643-1649.doi: 10.4103/1673-5374.167764

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

胶质母细胞瘤囊液促进坐骨神经再生?

  

  • 收稿日期:2015-08-20 出版日期:2015-10-28 发布日期:2015-10-28

Does glioblastoma cyst fluid promote sciatic nerve regeneration?

Rafet Özay1, Abit Aktaş2, Mevlüt Özgür Taşkapılıoğlu3, Bora Gürer4, *, Bülent Erdoğan5, Yusuf Şükrü Çağlar6   

  1. 1 Department of Neurosurgery, Diskapi Y?ld?r?m Beyaz?t Educational and Research Hospital, Ankara, Turkey
    2 Department of Histology and Embryology, Faculty of Veterinary Medicine, Istanbul University, Istanbul, Turkey
    3 Department of Neurosurgery, School of Medicine, Uluda? University, Bursa, Turkey
    4 Fatih Sultan Mehmet Education and Research Hospital, Istanbul, Turkey
    5 Department of Neurosurgery, School of Medicine, Fatih University, Ankara, Turkey
    6 Department of Neurosurgery, School of Medicine, Ankara University, Ankara, Turkey
  • Received:2015-08-20 Online:2015-10-28 Published:2015-10-28

摘要:

胶质母细胞瘤囊液含有多种生长因子和细胞外基质蛋白,这些物质具有神经营养和促进神经生长因作用,因此我们假设其可促进损伤周围神经的再生。为了验证这一点,实验在横断大鼠坐骨神经后行神经外膜吻合,在吻合口外周包裹浸透胶质母细胞瘤囊液或生理盐水的可吸收性明胶海绵。神经功能和组织形态学检测发现胶质母细胞瘤囊液局部给药大鼠坐骨神经功能情况明显好于生理盐水局部干预大鼠,胶质母细胞瘤囊液包裹下的坐骨神经胶质瘢痕形成更少,轴突面积、数量、直径及神经纤维直径更多、更大,说明胶质母细胞瘤囊液有促进损伤坐骨神经再生的作用以及未来可应用的前景。

关键词: 神经再生, 周围神经损伤, 坐骨神经损伤, 囊液, 胶质母细胞瘤, 生长因子

Abstract:

Glioblastoma cyst fluid contains growth factors and extracellular matrix proteins which are known as neurotrophic and neurite-promoting agents. Therefore, we hypothesized that glioblastoma cyst fluid can promote the regeneration of injured peripheral nerves. To validate this hypothesis, we transected rat sciatic nerve, performed epineural anastomosis, and wrapped the injured sciatic nerve with glioblastoma cyst fluid- or saline-soaked gelatin sponges. Neurological function and histomorphological examinations showed that compared with the rats receiving local saline treatment, those receiving local glioblastoma cyst fluid treatment had better sciatic nerve function, fewer scars, greater axon area, counts and diameter as well as fiber diameter. These findings suggest that glioblastoma cyst fluid can promote the regeneration of injured sciatic nerve and has the potential for future clinical application in patients with peripheral nerve injury.

Key words: nerve regeneration, peripheral nerve injury, sciatic nerve injury, cyst fluid, glioblastoma, growth factors, neural regeneration