中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (10): 1650-1655.doi: 10.4103/1673-5374.167765

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

脊髓电刺激可保护周围神经损伤后位于脊髓背根和前角的神经元

  

  • 收稿日期:2015-07-29 出版日期:2015-10-28 发布日期:2015-10-28
  • 基金资助:

    临沂市科技重点项目(201313026)

Pulsed electrical stimulation protects neurons in the dorsal root and anterior horn of the spinal cord after peripheral nerve injury

Bao-an Pei1, Jin-hua Zi1, Li-sheng Wu1, *, Cun-hua Zhang1, Yun-zhen Chen2, *   

  1. 1 Second Department of Traumatology, Linyi People’s Hospital, Linyi, Shandong Province, China
    2 Department of Orthopedics, Qilu Hospital, Shandong University, Jinan, Shandong Province, China
  • Received:2015-07-29 Online:2015-10-28 Published:2015-10-28
  • Contact: Li-sheng Wu or Yun-zhen Chen,linyicsgk@126.com or qilucyz@163.com.
  • Supported by:

    This study was supported by the Key Scientific and Technological Program of Linyi City of China, No. 201313026.

摘要:

针对周围神经损伤的研究大都局限在对损伤局部的修复与刺激,而对近端神经元胞体的研究相对较少。实验在大鼠坐骨结节处取下长约10mm的神经,将其近远端倒置后,行T10和L3脉冲电刺激,电流强度6.5 mA/h,刺激频率15Hz,15min/次,2次/d,连续56d。结果显示经脊髓脉冲电刺激后,坐骨神经损伤大鼠背根神经节神经元及前角神经元数量增加,坐骨神经中有髓神经纤维数量增加,背根神经节神经元和脊髓神经元超微结构明显改善,坐骨神经传导速度提升。提示脊髓脉冲电刺激对周围神经损伤后位于背根神经节的中枢感觉神经元和位于脊髓前角的中枢运动神经元均具有保护作用,从而促进周围神经纤维的再生。

关键词: 神经再生, 周围神经, 脉冲电刺激, 脊髓, 神经元, 背根神经节, 神经传导

Abstract:

Most studies on peripheral nerve injury have focused on repair at the site of injury, but very few have examined the effects of repair strategies on the more proximal neuronal cell bodies. In this study, an approximately 10-mm-long nerve segment from the ischial tuberosity in the rat was transected and its proximal and distal ends were inverted and sutured. The spinal cord was subjected to pulsed electrical stimulation at T10 and L3, at a current of 6.5 mA and a stimulation frequency of 15 Hz, 15 minutes per session, twice a day for 56 days. After pulsed electrical stimulation, the number of neurons in the dorsal root ganglion and anterior horn was increased in rats with sciatic nerve injury. The number of myelinated nerve fibers was increased in the sciatic nerve. The ultrastructure of neurons in the dorsal root ganglion and spinal cord was noticeably improved. Conduction velocity of the sciatic nerve was also increased. These results show that pulsed electrical stimulation protects sensory neurons in the dorsal root ganglia as well as motor neurons in the anterior horn of the spinal cord after peripheral nerve injury, and that it promotes the regeneration of peripheral nerve fibers.

Key words: nerve regeneration, peripheral nerve, pulsed electrical stimulation, spinal cord, neurons, dorsal root ganglion, nerve conduction, neural regeneration