中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (1): 156-162.doi: 10.4103/1673-5374.175063

• 观点:神经损伤修复保护与再生 • 上一篇    下一篇

老药脑活素改善了糖尿病模型小鼠受损坐骨神经的功能

  

  • 收稿日期:2015-07-07 出版日期:2016-01-15 发布日期:2016-01-15

Cerebrolysin improves sciatic nerve dysfunction in a mouse model of diabetic peripheral neuropathy

Han-yu Dong 1, Xin-mei Jiang 2, Chun-bo Niu 3, Lin Du 1, Jun-yan Feng 1, Fei-yong Jia 1, 2   

  1. 1 Department of Pediatric Neurology and Rehabilitation, First Hospital of Jilin University, Changchun, Jilin Province, China
    2 Institute of Jilin Neurological Research, First Hospital of Jilin University, Changchun, Jilin Province, China
    3 Department of Pathology, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China
  • Received:2015-07-07 Online:2016-01-15 Published:2016-01-15
  • Contact: Fei-yong Jia, Ph.D.,erkekangfujia@163.com.

摘要:

为验证老药脑活素对糖尿病模型小鼠周围神经病变的治疗效果,实验以高糖高脂饲料联合链脲佐霉素腹腔注射建立2型糖尿病小鼠模型,分别以1.80,5.39和8.98 mL/kg脑活素腹腔注射连续治疗10d。发现经脑活素治疗的糖尿病小鼠坐骨神经中有髓神经纤维数量、直径和面积增加,且行为学测试中斜板角度明显增加,甩尾潜伏期延长,舔足行为时间延长,坐骨神经功能指数增加,作用呈剂量依赖性。结局数据说明脑活素能够减轻糖尿病小鼠周围神经的病变。

关键词: 神经再生, 周围神经病变, 糖尿病, 脑活素, 神经功能, 坐骨神经

Abstract:

To examine the effects of Cerebrolysin on the treatment of diabetic peripheral neuropathy, we first established a mouse model of type 2 diabetes mellitus by administering a high-glucose, high-fat diet and a single intraperitoneal injection of streptozotocin. Mice defined as diabetic in this model were then treated with 1.80, 5.39 or 8.98 mL/kg of Cerebrolysin via intraperitoneal injections for 10 consecutive days. Our results demonstrated that the number, diameter and area of myelinated nerve fibers increased in the sciatic nerves of these mice after administration of Cerebrolysin. The results of several behavioral tests showed that Cerebrolysin dose-dependently increased the slope angle in the inclined plane test (indicating an improved ability to maintain body position), prolonged tail-flick latency and foot-licking time (indicating enhanced sensitivity to thermal and chemical pain, respectively, and reduced pain thresholds), and increased an index of sciatic nerve function in diabetic mice compared with those behavioral results in untreated diabetic mice. Taken together, the anatomical and functional results suggest that Cerebrolysin ameliorated peripheral neuropathy in a mouse model of type 2 diabetes mellitus.

Key words: nerve regeneration, peripheral neuropathy, diabetes mellitus, Cerebrolysin, neurological function, sciatic nerve, neural regeneration