中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (2): 270-277.doi: 10.4103/1673-5374.177735

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

野菊花乙醇提取物保护脑缺血再灌注损伤的抗炎效应

  

  • 收稿日期:2015-09-28 出版日期:2016-02-15 发布日期:2016-02-15
  • 基金资助:

    韩国科学部，ICT和未来计划基础科学研究项目(NRF-2013R1A2A2A01068190)；Hallym大学专项基金(HRF-S-13)

Neuroprotection of Chrysanthemum indicum Linne against cerebral ischemia/reperfusion injury by anti-inflammatory effect in gerbils

Ki-Yeon Yoo 1, In Hye Kim 2, Jeong-Hwi Cho 2, Ji Hyeon Ahn 2, Joon Ha Park 2, Jae-Chul Lee 2, Hyun-Jin Tae 3, Dae Won Kim 4,Jong-Dai Kim 5, Seongkweon Hong 6, Moo-Ho Won 2, Il Jun Kang 7   

  1. 1 Department of Oral Anatomy, College of Dentistry and Research Institute of Oral Science, Gangneung-Wonju National University, Gangneung, Republic of Korea
    2 Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea
    3 Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, Republic of Korea
    4 Department of Biochemistry and Molecular Biology, College of Dentistry and Research Institute of Oral Science, Gangneung-Wonju National University, Gangneung, Republic of Korea
    5 Division of Food Biotechnology, School of Biotechnology, Kangwon National University, Chuncheon, Republic of Korea
    6 Department of Surgery, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea
    7 Department of Food Science and Nutrition, Hallym University, Chuncheon, Republic of Korea
  • Received:2015-09-28 Online:2016-02-15 Published:2016-02-15
  • Contact: Moo-Ho Won, DVM, Ph.D. or Il-Jun Kang, Ph.D.,mhwon@kangwon.ac.k or ijkang@hallym.ac.kr.
  • Supported by:

    This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2013R1A2A2A01068190), and by Hallym University Specialization Fund (HRF-S-13).

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摘要：

野菊花作为一种在东亚地区广泛使用的植物药，其抗氧化和抗炎作用已得到证实，但其是否对大脑缺血再灌注有神经保护作用尚不清楚。由此，实验在以双侧颈总动脉阻塞法建立脑缺血再灌注沙鼠模型前喂饲野菊花乙醇提取物连续7d，NeuN免疫组化和Fluoro-Jade B组织荧光染色显示，野菊花乙醇提取物可减轻缺血海马CA1区锥体神经元损害，使缺血海马CA1区抗炎因子白细胞介素13的免疫反应升高或维持在较高水平，这也得到了免疫印迹检测结果的证实。实验结果说明野菊花乙醇提取物预处理通过抗炎途径，对脑缺血再灌注起神经保护作用。

关键词: 神经再生, 脑缺血再灌注, 神经元死亡, 海马, 锥体神经元, 炎症因子

Abstract:

In this study, we tried to verify the neuroprotective effect of Chrysanthemum indicum Linne (CIL) extract, which has been used as a botanical drug in East Asia, against ischemic damage and to explore the underlying mechanism involving the anti-inflammatory approach. A gerbil was given CIL extract for 7 consecutive days followed by bilateral carotid artery occlusion to make a cerebral ischemia/reperfusion model. Then, we found that CIL extracts protected pyramidal neurons in the hippocampal CA1 region (CA1) from ischemic damage using neuronal nucleus immunohistochemistry and Fluoro-Jade B histofluorescence. Accordingly, interleukin-13 immunoreactivities in the CA1 pyramidal neurons of CIL-pretreated animals were maintained or increased after cerebral ischemia/reperfusion. These findings indicate that the pre-treatment of CIL can attenuate neuronal damage/death in the brain after cerebral ischemia/reperfusion via an anti-inflammatory
approach.

Key words: nerve regeneration, transient cerebral ischemia, delayed neuronal death, pyramidal neurons, inflammatory cytokines, neural regeneration