中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (2): 305-311.doi: 10.4103/1673-5374.177740

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

针刺外关穴的神经保护作用?

  

  • 收稿日期:2015-07-30 出版日期:2016-02-15 发布日期:2016-02-15
  • 基金资助:

    国家自然科学基金(81273672, 81373720);福建省自然科学基金(2014J01353);福建省卫生系统青年骨干人才培养项目(2014-ZQN-JC-28)

Manual acupuncture at the SJ5 (Waiguan) acupoint shows neuroprotective effects by regulating expression of the anti-apoptotic gene Bcl-2

Dong Lin 1, Li-li Lin 1, Kyle Sutherland 2, Chuan-hai Cao 2   

  1. 1 College of Acupuncture, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian Province, China
    2 Department of Neurosurgery and Brain Repair, Morsani College of Medicine, University of South Florida, Tampa, FL, USA
  • Received:2015-07-30 Online:2016-02-15 Published:2016-02-15
  • Contact: Chuan-hai Cao, M.D.,ccao@health.usf.edu.
  • Supported by:

    This work was supported by the National Natural Science Foundation of China, No. 81273672, 81373720; the Natural Science Foundation of Fujian Province of China, No. 2014J01353; and a grant from Fujian Province Health Planning Commission Project (2014) for Training Young Talents, No. 2014-ZQN-JC-28.

摘要:

针刺外关穴已被证实对脑梗死有神经保护作用,但其确切作用机制尚待研究。实验应用Oligo GEarray™系统对正常大鼠小脑基因表达进行分析,筛选出113个与18条信号通路相关的基因。20min的针刺外关穴干预后,real-time PCR检测到大鼠小脑Bcl-2, Birc1b基因表达明显上调,免疫印迹检测到Bcl-2蛋白的表达至干预后2h仍维持在一个较高水平。说明针刺可调节抗凋亡基因Bcl-2的表达,从而起到潜在的神经保护作用。

关键词: 神经再生, 神经保护, 针刺, 外关, Bcl-2, 小脑, 凋亡, 信号通路

Abstract:

Acupuncture at the SJ5 (Waiguan) acupoint has neuroprotective effects in cerebral infarction, but the underlying mechanism remains poorly understood. Here, we analyzed gene expression in healthy rat cerebellum using a pathway-focused DNA microarray to screen 113 genes associated with 18 signal transduction pathways. After 20 minutes of acupuncture at SJ5, the expression of Bcl-2 and Birc1b mRNA was markedly increased. This was confirmed by real-time reverse transcription PCR. Furthermore, western blot analysis showed that Bcl-2 protein expression remained high in the cerebellum until at least 2 hours after cessation of acupuncture. These findings indicate that acupuncture at SJ5 exerts neuroprotective effects by regulating the expression of anti-apoptotic gene Bcl-2.

Key words: nerve regeneration, neuroprotection, acupuncture, SJ5, cerebellum, apoptosis, signal transduction, Bcl-2, neural regeneration