中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (3): 441-446.doi: 10.4103/1673-5374.179056

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

毛冬青总黄酮干预短暂性脑缺血发作:可减轻神经元损伤和病变程度

  

  • 收稿日期:2015-10-20 出版日期:2016-03-15 发布日期:2016-03-15
  • 基金资助:

    国家“重大新药创制”科技重大专项(2009ZX09103-324);河南省高校科技创新团队(2012IRTSTHN011)

Radix Ilicis Pubescentis total flavonoids ameliorates neuronal damage and reduces lesion extent in a mouse model of transient ischemic attack

Ming-san Miao, Lin Guo, Rui-qi Li, Xiao-lei Zhang   

  1. College of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan Province, China
  • Received:2015-10-20 Online:2016-03-15 Published:2016-03-15
  • Contact: Ming-san Miao, M.D.,miaomingsan@126.com
  • Supported by:

    This study was supported by the State “Major New Drug Creation” Major Science and Technology Project of China, No. 2009ZX09103-324; a grant from the Henan Provincial Science and Technology Innovation Team in University in China, No. 2012IRTSTHN011.

摘要:

毛冬青总黄酮是中药毛冬青的主要成分,前期研究显示其对脑缺血有一定保护作用。实验假设其有预防短暂性脑缺血发生的作用,故以过氧化叔丁醇诱发小鼠短暂性脑缺血过程造模,在损伤前3d起,以0.3,0.15,0.075 g/kg的剂量每天给予毛冬青总黄酮灌胃给药,共10d。以分光光度法检测其可增强机体清除氧自由基的能力,减缓脑损伤的病变过程;苏木精-伊红染色结果发现其可减轻小鼠短暂性脑缺血模型脑神经元和脑部血管损伤程度。由此认为,毛冬青总黄酮可能通过抗氧化作用,预防性减轻短暂性脑缺血发作造成的脑组织损害。

关键词: 神经再生, 短暂性脑缺血发作, 毛冬青总黄酮, 过氧化叔丁醇, 丙二醛, 一氧化氮, 一氧化氮合成酶, 超氧化物歧化酶

Abstract:

Flavonoids are a major component in the traditional Chinese medicine Radix Ilicis Pubescentis. Previous studies have shown that the administration of Radix Ilicis Pubescentis total flavonoids is protective in cerebral ischemia. However, to our knowledge, no studies have examined whether the total flavonoids extracted from Radix Ilicis Pubescentis prevent or ameliorate neuronal damage following transient ischemic attacks. Therefore, Radix Ilicis Pubescentis total flavonoids question and the potential underlying mechanisms. Thus, beginning 3 days before the induction of a mouse model of transient ischemic attack using tert-butyl hydroperoxide injections, mice were intragastrically administered 0.3, 0.15, or 0.075 g/kg of Radix Ilicis Pubescentis total flavonoids daily for 10 days. The results of spectrophotometric analyses demonstrated that Radix Ilicis Pubescentis total flavonoids enhanced oxygen free radical scavenging and reduced pathological alterations in the brain. Hematoxylin-eosin staining results showed that Radix Ilicis Pubescentis total flavonoids reduced hippocampal neuronal damage and cerebral vascular injury in this mouse model of transient ischemic attack. These results suggest that the antioxidant effects of Radix Ilicis Pubescentis total flavonoids alleviate the damage to brain tissue caused by transient ischemic attack.

Key words: nerve regeneration, transient ischemic attack, Radix Ilicis Pubescentis total flavonoids, tert-butyl hydroperoxide, malondialdehyde, nitric oxide, nitric oxide synthetase, superoxide dismutase, neural regeneration