中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (12): 2004-2011.doi: 10.4103/1673-5374.197145

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

电针督脉穴位保护损伤脊髓:Wnt/β-catenin通路的作用

  

  • 收稿日期:2016-11-24 出版日期:2016-12-31 发布日期:2016-12-31
  • 基金资助:
    国家自然科学基金(81373728)

Electroacupuncture at Dazhui (GV14) and Mingmen (GV4) protects against spinal cord injury: the role of the Wnt/β-catenin signaling pathway

Xin Wang1, #, Su-hua Shi2, #, Hai-jiang Yao3, Quan-kai Jing1, Yu-ping Mo4, Wei Lv1, Liang-yu Song1, Xiao-chen Yuan5, Zhi-gang Li1, *, Li-na Qin2, *   

  1. 1 School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China 2 Department of Rehabilitation, Te Tird Afliated Hospital of Beijing University of Chinese Medicine, Beijing, China 3 Treatment Center of Traditional Chinese Medicine, Beijing Bo’ai Hospital, Chinese Rehabilitation Research Center, School of Rehabilitation Medicine, Capital Medical University, Beijing, China 4 Department of Rehabilitation, Te Tird People’s Hospital of Shenzhen, Shenzhen, Guangdong Province, China 5 Institute of Microcirculation, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China
  • Received:2016-11-24 Online:2016-12-31 Published:2016-12-31
  • Contact: Zhi-gang Li or Li-na Qin, lizhigang620@126.com or qinqin1978119@sina.com.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, No. 81373728.

摘要:

电针督脉大椎和命门对脊髓损伤的治疗作用已得到证实,但其具体作用机制仍有待进一步研究。为研究电针督脉对脊髓损伤的作用机制,实验应用重物自由落体打击法建立脊髓损伤大鼠模型,给予其电针大椎穴和命门穴治疗,1次/d,20min/次。发现干预1,7,14d后,BBB运动功能评分显示电针脊髓损伤大鼠运动功能明显改善,HE染色显示脊髓组织病理改变明显减轻,免疫组化和免疫印迹检测到损伤脊髓组织中Wnt-1, Wnt-3A,β-catenin免疫反应及蛋白表达明显上调。说明电针督脉大椎和命门可通过上调Wnt/β-catenin信号通路中的Wnt-1, Wnt-3A,β-catenin表达,对脊髓损伤起到神经保护作用。 

orcid: 0000-0003-0378-3496 (Zhi-gang Li), 0000-0002-6646-249X (Li-na Qin) 

关键词: 神经再生, 脊髓损伤, 电针, 督脉, 大椎, 命门, Wnt/β-catenin信号通路, 神经保护

Abstract: lectroacupuncture at Dazhui (GV14) and Mingmen (GV4) on the Governor Vessel has been shown to exhibit curative effects on spinal cord injury; however, the underlying mechanism remains poorly understood. In this study, we established rat models of spinal cord injury using a modifed Allen’s weight-drop method. Ninety-nine male Sprague-Dawley rats were randomly divided into three equal groups: sham (only laminectomy), SCI (induction of spinal cord injury at T10), and EA (induction of spinal cord injury at T10 and electroacupuncture intervention at GV14 and GV4 for 20 minutes once a day). Rats in the SCI and EA groups were further randomly divided into the following subgroups: 1-day (n = 11), 7-day (n = 11), and 14-day (n = 11). At 1, 7, and 14 days afer electroacupuncture treatment, the Basso, Beattie and Bresnahan locomotor rating scale showed obvious improvement in rat hind limb locomotor function, hematoxylin-eosin staining showed that the histological change of injured spinal cord tissue was obviously alleviated, and immunohistochemistry and western blot analysis showed that Wnt1, Wnt3a, β-catenin immunoreactivity and protein expression in the injured spinal cord tissue were greatly increased compared with the sham and SCI groups. Tese fndings suggest that electroacupuncture at GV14 and GV4 upregulates Wnt1, Wnt3a, and β-catenin expression in the Wnt/β-catenin signaling pathway, exhibiting neuroprotective effects against spinal cord injury.

Key words: nerve regeneration, spinal cord injury, electroacupuncture, Governor Vessel, Dazhui (GV14) acupoint, Mingmen (GV4) acupoint, Wnt/β-cateninsignaling pathway, neuroprotection, neural regeneration