中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2017, Vol. 12 ›› Issue (2): 228-234.doi: 10.4103/1673-5374.200806

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

电针干预通过介导腺苷A1受体对局灶性脑缺血发挥神经保护

  

  • 收稿日期:2017-01-12 出版日期:2017-02-15 发布日期:2017-02-15
  • 基金资助:

    中国国家自然科学基金项目（81273926，81573742），浙江省自然科学基金项目（LY15H290006）

Electroacupuncture-induced neuroprotection against focal cerebral ischemia in the rat is mediated by adenosine A1 receptors

Qin-xue Dai1, Wu-jun Geng1, Xiu-xiu Zhuang1, Hong-fa Wang1, Yun-chang Mo1, He Xin1, Jiang-fan Chen2, Jun-lu Wang1   

  1. 1 Department of Anesthesiology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China; 
    2 Department of Neurology, Boston University School of Medicine, Boston, MA, USA
  • Received:2017-01-12 Online:2017-02-15 Published:2017-02-15
  • Contact: Jun-lu Wang,wangjunlu973@163.com.
  • Supported by:

    This work was supported by the National Natural Science Foundation of China, No. 81273926, 81573742; the Natural Science Foundation
    of Zhejiang Province of China, No. LY15H290006.

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摘要：

腺苷A1受体的活化对缺血性脑损伤可起保护作用，而电针预处理已被证明可减轻缺血性脑损伤，为验证其是否与此相关。实验首先对正常大鼠行30 min的电针百会（电流为1 mA，频率为2/15Hz，深度1mm），高性能液相色谱分析，发现电刺激结束15和120min时，大脑皮质ATP和腺苷水平分别达到峰值；在电针刺激大鼠百会30min预处理完成后15或120min行大脑中动脉闭塞建立脑缺血模型，发现再灌注24h后，120min前预处理的大鼠脑梗死体积及神经功能明显改善，而15min前预处理则无此效果；而后对大鼠腹腔注射1 mg/kg腺苷A1受体拮抗剂8-环戊烷基-1,3-二丙基黄嘌呤30min后，行电针刺激百会30min预处理，再经120min行大脑中动脉闭塞致脑缺血，发现电针的脑保护作用消失。结果表明电针百会预处理对脑缺血的保护作用是通过腺苷A1受体实现的。

ORCID:0000-0002-1525-3938(Jun-lu Wang)

关键词: 神经再生, 腺苷, ATP , 腺苷A1受体, 局灶性脑缺血, 电针, 预处理, DPCPX , 高效液相色谱

Abstract:

The activation of adenosine A1 receptors is important for protecting against ischemic brain injury and pretreatment with electroacupuncture has been shown to mitigate ischemic brain insult. The aim of this study was to test whether the adenosine A1 receptor mediates electroacupuncture pretreatment-induced neuroprotection against ischemic brain injury. We first performed 30 minutes of electroacupuncture pretreatment at the Baihui acupoint (GV20), delivered with a current of 1 mA, a frequency of 2/15 Hz, and a depth of 1 mm. High-performance liquid chromatography found that adenosine triphosphate and adenosine levels peaked in the cerebral cortex at 15 minutes and 120 minutes after electroacupuncture pretreatment, respectively. We further examined the effect of 15 or 120 minutes electroacupuncture treatment on ischemic brain injury in a rat middle cerebral artery-occlusion model. We found that at 24 hours reperfusion,120 minutes after electroacupuncture pretreatment, but not for 15 minutes, significantly reduced behavioral deficits and infarct volumes. Last, we demonstrated that the protective effect gained by 120 minutes after electroacupuncture treatment before ischemic injury was abolished by pretreatment with the A1-receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine (1 mg/kg, intraperitoneally). Our results suggest that pretreatment with electroacupuncture at the Baihui acupoint elicits protection against transient cerebral ischemia via action at adenosine A1 receptors.

Key words: nerve regeneration, adenosine, adenosine triphosphate, adenosine A1 receptor, cerebral ischemia, electroacupuncture, pretreatment, 8-cyclopentyl-1,3-dipropylxanthine, high-performance liquid chromatography, neural regeneration