中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (3): 477-483.doi: 10.4103/1673-5374.228731

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

电针和艾灸促进损伤坐骨神经再生的途径：许旺细胞增殖和神经生长因子分泌

  

  • 收稿日期:2018-01-13 出版日期:2018-03-15 发布日期:2018-03-15
  • 基金资助:

    国家自然科学基金（81373754, 81102670）

Electroacupuncture and moxibustion promote regeneration of injured sciatic nerve through Schwann cell proliferation and nerve growth factor secretion

Lin-na Hu1, Jin-xin Tian1, 2, Wei Gao1, 3, Jing Zhu1, Fang-fang Mou1, Xiao-chun Ye1, Yu-pu Liu1, Ping-ping Lu1, Shui-jin Shao1,Hai-dong Guo1   

  1. 1 Department of Anatomy, School of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China
    2 Department of Internal Medicine, Shanghai Changhang Hospital, Shanghai, China
    3 Department of Gynaecology and Obstetrics, Heze Hospital of Traditional Chinese Medicine, Heze, Shandong Province, China
  • Received:2018-01-13 Online:2018-03-15 Published:2018-03-15
  • Contact: Shui-jin Shao, Ph.D. or Hai-dong Guo, Ph.D.,shaoshuijin@163.com or hdguo8@hotmail.com.
  • Supported by:

    This work was supported by the National Natural Science Foundation of China, No. 81373754, 81102670

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摘要：

电针和艾灸疗法治疗周围神经损伤疗效较好且不良反应少，但其神经修复机制尚不清楚的问题。实验采用体内和体外实验拟进一步揭示电针和艾灸促进损伤周围神经再生的作用及途径，以神经切断法建立坐骨神经损伤大鼠模型，分别对其进行电针或艾灸干预，干预穴位为环跳(GB30)和足三里(ST36)，每次干预15 min，每周6次，连续治疗4周。以行为学检测方法观察坐骨神经指数变化，以电生理检测观察坐骨神经传导速度变化，以苏木精伊红染色检测腓肠组织变化，以免疫组织化学染色检测神经组织许旺细胞标志物-S-100的免疫反应变化，酶联免疫吸附测定血清神经生长因子表达水平。结果发现，与模型组相比，电针和艾灸组大鼠坐骨神经功能指数、传导速度恢复率、腓肠肌肌细胞直径恢复率、损伤神经组织S-100阳性细胞数及血清神经生长因子表达水平均升高，且电针和艾灸组干预效果相近。实验又收集了各组大鼠的血清对体外培养的许旺细胞进行处理，发现电针血清组和艾灸血清组中许旺细胞活力在处理后3，5 d明显高于模型血清组。上述数据表明，电针和艾灸均可促进损伤后坐骨神经再生和功能恢复，其机制可能与其促进许旺细胞增殖和上调神经生长因子表达有关。

orcid: 0000-0001-9340-5426(Shui-jin Shao)
0000-0001-9346-6819(Hai-dong Guo)

关键词: 周围神经损伤, 电针, 艾灸, 针刺, 血清, 神经再生, Schwann细胞, 神经生长因子, 增殖, 再生, 坐骨神经功能指数

Abstract:

Using electroacupuncture and moxibustion to treat peripheral nerve injury is highly efficient with low side effects. However, the electroacupuncture-and moxibustion-based mechanisms underlying nerve repair are still unclear. Here, in vivo and in vitro experiments uncovered one mechanism through which electroacupuncture and moxibustion affect regeneration after peripheral nerve injury. We first established rat models of sciatic nerve injury using neurotomy. Rats were treated with electroacupuncture or moxibustion at acupoints Huantiao (GB30) and Zusanli (ST36). Each treatment lasted 15 minutes, and treatments were given six times a week for 4 consecutive weeks. Behavioral testing was used to determine the sciatic functional index. We used electrophysiological detection to measure sciatic nerve conduction velocity and performed hematoxylin-eosin staining to determine any changes in the gastrocnemius muscle. We used immunohistochemistry to observe changes in the expression of S100—a specific marker for Schwann cells—and an enzyme-linked immunosorbent assay to detect serum level of nerve growth factor. Results showed that compared with the model-only group, sciatic functional index, recovery rate of conduction velocity, diameter recovery of the gastrocnemius muscle fibers, number of S100-immunoreactive cells,and level of nerve growth factor were greater in the electroacupuncture and moxibustion groups. The efficacy did not differ between treatment groups. The serum from treated rats was collected and used to stimulate Schwann cells cultured in vitro. Results showed that the viability of Schwann cells was much higher in the treatment groups than in the model group at 3 and 5 days after treatment. These findings indicate that electroacupuncture and moxibustion promoted nerve regeneration and functional recovery; its mechanism might be associated with the enhancement of Schwann cell proliferation and upregulation of nerve growth factor.

Key words: nerve regeneration, peripheral nerve injury, electroacupuncture, moxibustion, acupuncture, serum, Schwann cells, nerve growth factor, proliferation, regeneration, sciatic functional index, neural regeneration