中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2017, Vol. 12 ›› Issue (7): 1103-1110.doi: 10.4103/1673-5374.211189

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

人华通胶间充质干细胞移植后可神经分化促进缺血性脑卒中大鼠神经功能恢复

  

  • 收稿日期:2017-06-10 出版日期:2017-07-15 发布日期:2017-07-15
  • 基金资助:

    中国国家自然科学基金项目（31171038），江苏省自然科学基金项目（BK2011385），江苏省333项目（BRA2016450），南通大学大学生创新创业培训项目（201510304033Z, 201610304053Z），南通大学毕业生创新创业培训项目（YKC14050, YKC15046），江苏省高校重点学科建设项目

Neural differentiation of human Wharton’s jelly-derived mesenchymal stem cells improves the recovery of neurological function after transplantation in ischemic stroke rats

Lei Zhang1, Lin-mei Wang1, Wei-wei Chen2, Zhi Ma1, Xiao Han1, Cheng-ming Liu1, Xiang Cheng1, Wei Shi3, Jing-jing Guo1, Jian-bing Qin1, Xiao-qing Yang4, Guo-hua Jin1, 5, Xin-hua Zhang1, 5   

  1. 1 Department of Anatomy, Nantong University, Nantong, Jiangsu Province, China; 2 Department of Radiation Oncology, Third People’s Hospital of Yancheng, Yancheng, Jiangsu Province, China; 3 Department of Neurosurgery, the Affiliated Hosptial of Nantong University, Nantong, Jiangsu Province, China; 4 Department of Obstetrics and Gynecology, the Affiliated Hosptial of Nantong University, Nantong, Jiangsu Province, China; 5 Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China
  • Received:2017-06-10 Online:2017-07-15 Published:2017-07-15
  • Contact: Xin-hua Zhang, Ph.D., zhangxinhua@ntu.edu.cn.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 31171038; the Natural Science Foundation of Jiangsu Province of China, No. BK2011385; the “333” Program Funding of Jiangsu Province of China, No. BRA2016450; the Training Program of Innovation and Entrepreneurship for Undergraduates of Nantong University of China, No. 201510304033Z, 201610304053Z; the Training Program of Innovation and Entrepreneurship for Graduates of Nantong University of China, No. YKC14050, YKC15046; and a grant from Funds for the Priority Academic Program Development of Jiangsu Higher Education Institutions of China.

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摘要：

人华通胶间充质干细胞具有优异的增殖潜力、分化能力、低免疫原性，并且容易获取，但既往少见其在治疗缺血性脑卒中方面应用的报道，其治疗效果仍需实验验证。为此，实验设计阻塞大鼠大脑中动脉闭塞48h建立缺血性脑卒中模型，经尾静脉移植人华通胶间充质干细胞。4周后发现大鼠神经功能明显改善，人华通胶间充质干细胞更多地归巢到大鼠缺血额叶皮质中，并在此区域分化为神经元样细胞。结果表明人华通胶间充质干细胞能够在缺血性脑卒中大鼠缺血部位分化为神经元样细胞，进而改善其神经行为能力。

ORCID:0000-0002-5702-6733(Xin-hua Zhang)

关键词: 神经再生, 人华通胶间充质干细胞, 缺血性卒中, 细胞移植, 神经分化, 大脑中动脉闭塞, 神经功能

Abstract:

Human Wharton’s jelly-derived mesenchymal stem cells (hWJ-MSCs) have excellent proliferative ability, differentiation ability, low immunogenicity, and can be easily obtained. However, there are few studies on their application in the treatment of ischemic stroke, therefore their therapeutic effect requires further verification. In this study, hWJ-MSCs were transplanted into an ischemic stroke rat model via the tail vein 48 hours after transient middle cerebral artery occlusion. After 4 weeks, neurological functions of the rats implanted with hWJ-MSCs were significantly recovered. Furthermore, many hWJ-MSCs homed to the ischemic frontal cortex whereby they differentiated into neuron-like cells at this region. These results confirm that hWJ-MSCs transplanted into the ischemic stroke rat can differentiate into neuron-like cells to improve rat neurological function and behavior.

Key words: nerve regeneration, human Wharton’s jelly-derived mesenchymal stem cells, ischemic stroke, cell transplantation, middle cerebral artery occlusion, neural differentiation, neurological function, neural regeneration