中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2017, Vol. 12 ›› Issue (8): 1365-1374.doi: 10.4103/1673-5374.213693

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

渐进性脊髓压迫损伤小鼠模型：脊髓功能和病理变化

  

  • 收稿日期:2017-08-11 出版日期:2017-08-15 发布日期:2017-08-15
  • 基金资助:

    中国国家自然科学基金项目（31400824），江门市科技计划项目（2015751），暨南大学第一临床医学院科研培育专项基金项目（2013208）

A progressive compression model of thoracic spinal cord injury in mice: function assessment and pathological changes in spinal cord

Guo-dong Sun1, Yan Chen1, Zhi-gang Zhou1, Shu-xian Yang2, Cheng Zhong3, Zhi-zhong Li1, 4   

  1. 1 Department of Orthopedics, First Affiliated Hospital, Jinan University, Guangzhou, Guangdong Province, China;
    2 Biomedical Translational Research Institute and Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou, Guangdong Province, China;
    3 Department of Traumatology and Plastic Surgery, The Affiliated Jiangmen Traditional Chinese Medicine Hospital of Jinan University, Jiangmen, Guangdong Province, China;
    4 Department of Orthopedics, Heyuan People’s Hospital (Heyuan Affiliated Hospital of Jinan University), Heyuan, Guangdong Province, China
  • Received:2017-08-11 Online:2017-08-15 Published:2017-08-15
  • Contact: Zhi-zhong Li, Ph.D.,lizhizhongjd@163.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 31400824; a grant from the Science and Technology Program of Jiangmen City of China, No. 2015751; and the Scientific Research and Cultivating Foundation of the First Clinical Medical College of Jinan University of China, No. 2013208.

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摘要：

临床中近半数的脊髓损伤为脊柱慢性压迫等非创伤性损伤，其相关基础研究动物模型以大鼠为主，较少考虑小鼠。因此作者拟开发一种渐进性脊髓压迫损伤小鼠模型。实验设计在成年C57BL/6小鼠T9椎板中插入螺钉以压迫脊髓，且每2周暴露一次螺钉，旋入0.2mm，进一步压缩脊髓，持续2个月。可见渐进性脊髓压迫损伤小鼠BMS评分以及步态情况随时间明显恶化，且小鼠后肢功能障碍程度与脊髓压迫程度一致，同时小鼠脊髓前角运动神经元数量减少，星形胶质细胞和小胶质细胞逐渐增多。说明此种方法能够有效建立渐进性脊髓压迫损伤小鼠模型。

orcid:0000-0002-2306-7633(Zhi-zhong Li)

关键词: 神经再生, 渐进性脊髓压迫损伤, 病理学变化, BMS评分, 步态, 运动诱发电位, 星形胶质细胞, 小胶质细胞, 运动神经元, 后肢功能障碍

Abstract:

Non-traumatic injury accounts for approximately half of clinical spinal cord injury, including chronic spinal cord compression. However,previous rodent spinal cord compression models are mainly designed for rats, few are available for mice. Our aim is to develop a thoracic progressive compression mice model of spinal cord injury. In this study, adult wild-type C57BL/6 mice were divided into two groups: in the surgery group, a screw was inserted at T9 lamina to compress the spinal cord, and the compression was increased by turning it further into the canal (0.2 mm) post-surgery every 2 weeks up to 8 weeks. In the control group, a hole was drilled into the lamina without inserting a screw. The results showed that Basso Mouse Scale scores were lower and gait worsened. In addition, the degree of hindlimb dysfunction in mice was consistent with the degree of spinal cord compression. The number of motor neurons in the anterior horn of the spinal cord was reduced in all groups of mice, whereas astrocytes and microglia were gradually activated and proliferated. In conclusion, this progressive compression of thoracic spinal cord injury in mice is a preferable model for chronic progressive spinal cord compression injury.

Key words: nerve regeneration, progressive spinal cord compression injury, pathological changes, Basso Mouse Scale scores, gait, motor evoked potentials, astrocytes, microglia, motor neurons, hindlimb dysfunction, neural regeneration