中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2017, Vol. 12 ›› Issue (10): 1695-1702.doi: 10.4103/1673-5374.217371

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

德克萨斯红-葡聚糖胺神经示踪可以有效显示大鼠腰段初级神经传入纤维的中枢投射和联系

  

  • 收稿日期:2017-07-04 出版日期:2017-10-15 发布日期:2017-10-15
  • 基金资助:

    中国医学科学基金（14CXZ007）

Central projections and connections of lumbar primary afferent fibers in adult rats: effectively revealed using Texas red-dextran amine tracing

Shi-de Lin1, 2, Tao Tang1, Ting-bao Zhao2, Shao-jun Liu1   

  1. 1 State Key Laboratory of Proteomics, Department of Neurobiology, Beijing Institute of Basic Medical Sciences, Beijing, China
    2 Department of Spinal Cord Injury, the General Hospital of Jinan Military Command, Jinan, Shandong Province, China
  • Received:2017-07-04 Online:2017-10-15 Published:2017-10-15
  • Contact: Shao-jun Liu, Ph.D.,liusj@bmi.ac.cn.
  • Supported by:

    This study was supported by the Medical Sciences Foundation of China, No. 14CXZ007.

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摘要：

来自背根神经传入纤维的信号对于后肢运动调节十分重要，但既往通过银染、放射自显影，以及WGA-HRP、CB-HRP等束路示踪的方法未能很好地勾勒出背根神经的中枢投射和联系模式。为此，实验设计将3-kDa德克萨斯红-葡聚糖胺注射入成年大鼠L4背根神经中，共聚焦显微镜观察结果显示神经纤维及膨体结构主要分布于同侧T12-S4节段脊髓灰质后角、T10-L2节段的Clarke’s柱及L1-L5节段脊髓灰质前角。从L4背根神经投射来的神经膨体结构在L4脊髓节段中非常浓密，特别是在灰质后角第Ⅰ-Ⅲ板层中。而越远离L4脊髓节段，膨体的密度越小。各脊髓节段中神经膨体均主要分布于灰质第Ⅰ-Ⅳ板层中，中等量分布于第Ⅴ-Ⅶ板层中，而少量分布于第Ⅷ-Ⅹ板层中。而且这些膨体结构与脊髓灰质中NeuN阳性的神经元以及前角运动神经元的胞体和突起间存在紧密接触，形成了类似突触结构的解剖学联系。结果显示德克萨斯红-葡聚糖胺背根神经示踪确实有效的显示了背根神经的中枢投射和联系。

orcid:0000-0001-7318-7628(Shao-jun Liu)

关键词: 神经再生, 脊髓损伤, 背根神经, 中枢投射, 联系, 德克萨斯红-葡聚糖胺

Abstract:

Signals from lumbar primary afferent fibers are important for modulating locomotion of the hind-limbs. However, silver impregnation techniques, autoradiography, wheat germ agglutinin-horseradish peroxidase and cholera toxin B subunit-horseradish peroxidase cannot image the central projections and connections of the dorsal root in detail. Thus, we injected 3-kDa Texas red-dextran amine into the proximal trunks of L4 dorsal roots in adult rats. Confocal microscopy results revealed that numerous labeled arborizations and varicosities extended to the dorsal horn from T12–S4, to Clarke’s column from T10–L2, and to the ventral horn from L1–5. The  abeled varicosities at the L4 cord level were very dense, particularly in laminae I–III, and the density decreased gradually in more rostral and caudal segments. In addition, they were predominately distributed in laminae I–IV, moderately in laminae V–VII and sparsely in laminae VIII–X. Furthermore, direct contacts of lumbar afferent fibers with propriospinal neurons were widespread in gray matter. In conclusion, the projection and connection patterns of L4 afferents were illustrated in detail by Texas red-dextran amine-dorsal root tracing.

Key words: nerve regeneration, spinal cord injury, dorsal root, central projection, connection, Texas red-dextran amine, neural regeneration