中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (2): 257-264.doi: 10.4103/1673-5374.226396

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

热敏灸35min与15min减少局灶性脑缺血再灌注后细胞凋亡作用的差异

  

  • 收稿日期:2017-11-17 出版日期:2018-02-15 发布日期:2018-02-15
  • 基金资助:

    国家自然科学基金项目（81060305，81660819），江西省自然科学基金项目（2015BAB205068），江西省科技合作重点项目（20161BBH80053），江西省卫计委重点项目（2014Z003），江西省中医药大学自然科学基金项目（2014ZR018，2015jzzdxk024）

Comparison of the anti-apoptotic effects of 15- and 35-minute suspended moxibustion after focal cerebral ischemia/reperfusion injury

Ai-jiao Xiao1, Lin He1, Xin Ouyang2, Jie-min Liu1, Ming-ren Chen2   

  1. 1 School of Basic Medical Science, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi Province, China
    2 School of Moxibustion, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi Province, China
  • Received:2017-11-17 Online:2018-02-15 Published:2018-02-15
  • Contact: Ming-ren Chen, M.D.,chenmr928@163.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81060305 & 81660819; the Natural Science Foundation of Jiangxi Province of China, No. 2015BAB205068; Key Program for Science and Technology Cooperation Projects of Jiangxi Province of China, No. 20161BBH80053; a grant from the Key Project of Health Commission of Jiangxi Province of China, No. 2014Z003; and the Natural Science Foundation of Jiangxi University of Traditional Chinese Medicine of China, No. 2014ZR018 & 2015jzzdxk024.

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摘要：

热敏灸对脑缺血再灌注损伤有较好的神经保护作用，但其作用机制尚不清楚。传统的艾灸疗法以局部红热为度，治疗时间约为15 min，热敏灸疗法釆用消敏定量，一般需要治疗30 min-1 h。但艾灸35 min与15min对脑缺血再灌注大鼠究竟有何影响还不清楚。为此，实验建立了局灶性脑缺血/再灌注损伤成年大鼠模型，分别艾灸大椎穴35 min和15 min，然后以TTC染色检测大鼠梗死体积，苏木精-伊红染色及TUNEL染色检测损伤部位病理变化及神经元凋亡，Western blot检测损伤部位caspase-9，caspase-3， Bax和Bcl-2表达。客观数据显示与艾灸大椎15 min的大鼠相比，艾灸大椎35min的大鼠梗死体积更小，大脑皮质中神经元缺失更少，且大脑皮质中caspase-9，caspase-3和Bax表达减少，Bcl-2表达增加。结果表明，艾灸大椎35min对脑缺血再灌注损伤抗凋亡效应优于艾灸大椎15 min。

orcid:0000-0001-6575-7416(Ai-jiao Xiao)
          0000-0003-0708-1002(Ming-ren Chen)

关键词: 神经再生, 艾灸, 大脑中动脉闭塞, 脑缺血再灌注, 梗死体积, 细胞凋亡, Bcl-2, Bax, caspase-9, caspase-3

Abstract:

Heat-sensitive suspended moxibustion has a neuroprotective effect against focal cerebral ischemia/reperfusion injury, but the underlying mechanisms remain unclear. The duration of heat-sensitive suspended moxibustion (usually from 30 minutes to 1 hour) is longer than traditional suspended moxibustion (usually 15 minutes). However, the effects of 15- and 35-minute suspended moxibustion in rats with cerebral ischemia/reperfusion injury are poorly understood. In this study, we performed 15- or 35-minute suspended moxibustion at acupoint Dazhui (GV14) in an adult rat model of focal cerebral ischemia/reperfusion injury. Infarct volume was evaluated with the 2,3,5-triphenyltetrazolium chloride assay. Histopathological changes and neuronal apoptosis at the injury site were assessed by hematoxylin-eosin staining and terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Caspase-9 and caspase-3 expression at the injury site was detected using immunofluorescent staining. Bax and Bcl-2 expression at the injury site was assessed using western blot assay.In the 35-minute moxibustion group, infarct volume was decreased, neuronal apoptosis was reduced, caspase-9, caspase-3 and Bax expression was lower, and Bcl-2 expression was increased, compared with the 15-minute moxibustion group. Our findings show that 35-minute moxibustion has a greater anti-apoptotic effect than 15-minute moxibustion after focal cerebral ischemia/reperfusion injury.

Key words: nerve regeneration, suspended moxibustion, middle cerebral artery occlusion, cerebral ischemia/reperfusion injury, infarct volume, apoptosis, Bcl-2, Bax, caspase-9, caspase-3, neural regeneration, traditional Chinese medical therapy