中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (10): 1811-1819.doi: 10.4103/1673-5374.238712

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

三种可生物降解聚合物导管修复小鼠损伤坐骨神经：形态和功能结局的比较

  

  • 收稿日期:2018-07-11 出版日期:2018-10-15 发布日期:2018-10-15

Comparison of morphological and functional outcomes of mouse sciatic nerve repair with three biodegradable polymer conduits containing poly(lactic acid)

Fernanda Marques Pestana1, 3, Rui C.C. Domingues4, Júlia Teixeira Oliveira2, 3, Daniela F. P. A. Durço2, 3, Camila Oliveira Goulart2, 3, Henrique Rocha Mendonça2, 3, 5, Anne Caroline Rodrigues dos Santos2, 3, Natália Tavares de Campos2, 3, Beatriz Theodoro da Silva2, 3, Cristina Cardoso Pereira4, Cristiano Piacsek Borges4, Ana Maria Blanco Martinez1, 2, 3   

  1. 1 Pós Graduação em Ciências Morfológicas, Instituto de Ciências Biomédicas-UFRJ, Rio de Janeiro, RJ, Brazil
    2 Anatomia Patológica - Faculdade de Medicina – HUCFF -UFRJ, Rio de Janeiro, RJ, Brazil
    3 Laboratório de Neurodegeneração e Reparo - Faculdade de Medicina – HUCFF-UFRJ, Rio de Janeiro, RJ, Brazil
    4 Programa de Eng. Química, COPPE-UFRJ, Rio de Janeiro, Brazil
    5 Polo Universitário de Macaé, Laboratório Integrado de Produtos Bioativos e Biociências, Macaé, UFRJ, Brazil
  • Received:2018-07-11 Online:2018-10-15 Published:2018-10-15
  • Contact: Ana Maria Blanco Martinez,MD, PhD,anamartinez@hucff.ufrj.br.
  • Supported by:

    This work was supported by Grant 465656/2014-5 from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq),Brasil, and also supported by CAPES and FAPERJ, Brasil.

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摘要：

目前正在研究含有聚（乳酸）（PLA）的神经导管（NGC）作为自体移植物的替代物进行神经修复，其导致在被移除的神经支配的区域中的永久性功能损伤。仅由PLA制成或与其他聚合物组合制成的多种导管已成功用于神经修复，但未见研究比较其修复效果。实验设计拟通过使用由聚（乳酸）与或不与聚己内酯（PLA / PCL）或聚乙烯吡咯烷酮（PLA / PVP）结合制成的NGC来修复小鼠损伤坐骨神经。采用机械痛觉过敏分析、坐骨神经功能指数（SFI）和神经肌电图评估神经功能恢复。机械痛觉过敏分析显示PLA组比PLA / PVP和PLA / PCL组改善更快。在电生理学测定中，PLA组显示出比PLA / PCL和PLA / PVP组更高的振幅。然而，各组之间的SFI改善率相近。形态学上，PLA组显示出更多的血管形成，而神经纤维再生在各组之间相近。总之，仅PLA构成的导管对损伤周围神经的修复效果优于其他神经导管。

orcid:0000-0002-0821-5730(Ana Maria Blanco Martinez)

 

关键词: 坐骨神经, 神经套接, 可生物降解的导管, 聚（乳酸）, 神经再生

Abstract:

Poly(lactic acid) (PLA)-containing nerve guidance conduits (NGCs) are currently being investigated for nerve repair as an alternative to autograft, which leads to permanent functional impairment in the territory innervated by the removed nerve. Combination of polymers modifies the physical properties of the conduits, altering their nerve-guidance properties. Conduits made from PLA-only or combined with other polymers have been used successfully for nerve repair, but their efficiency has not been compared. We compared the morphological and functional outcomes of peripheral nerve repair by using NGCs made of poly(lactic acid) and combined or not with polycaprolactone (PLA/PCL) or polyvinylpyrrolidone (PLA/PVP). To assess the functional recovery, we employed a mechanical hyperalgesia analysis, sciatic functional index (SFI), and electroneuromyography. The mechanical hyperalgesia analysis showed that the PLA group improved more rapidly than the PLA/PVP and PLA/PCL groups; similarly, in the electroneuromyography assay, the PLA group exhibited higher amplitude than the PLA/PCL and PLA/PVP groups. However, the SFI improvement rates did not differ among the groups. Morphologically, the PLA group showed more vascularization, while the nerve fiber regeneration did not differ among the groups. In conclusion, the PLA-only conduits were superior to the other NGCs tested for nerve repair.

Key words: sciatic nerve, tubulization, biodegradable conduit, poly(lactic acid), regeneration