中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (10): 1820-1826.doi: 10.4103/1673-5374.238713

• 原著:颅神经损伤修复保护与再生 • 上一篇    下一篇

血管内皮生长因子联合血小板源性生长因子可促进小鼠单侧球囊切除后的嗅觉神经元再生

  

  • 收稿日期:2018-07-11 出版日期:2018-10-15 发布日期:2018-10-15
  • 基金资助:

    昆士兰科技大学启动资助（FC）和克莱姆琼斯基金会（给JASJ）资助

Combined VEGF/PDGF improves olfactory regeneration after unilateral bulbectomy in mice

Kate Beecher1, 3, Louise M. Hafner1, 2, Jenny Ekberg3, 4, James A. St. John3, 4, Fatemeh Chehrehasa1, 2, 3   

  1. 1 School of Biomedical Sciences, Queensland University of Technology, Box 2434, QLD, Australia
    2 Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Ave, Kelvin Grove, QLD, Australia
    3 Clem Jones Centre for Neurobiology and Stem Cell Research, Griffith Institute for Drug Discovery, Griffith University, 170 Kessels Rd, Nathan, Australia
    4 Menzies Health Institute Queensland, Griffith University, Southport 4222, QLD, Australia
  • Received:2018-07-11 Online:2018-10-15 Published:2018-10-15
  • Contact: Fatemeh Chehrehasa, PhD,fatemeh.chehrehasa@qut.edu.au.
  • Supported by:

    This work was supported by Queensland University of Technology Start Up Grant (to FC) and a grant from the Clem Jones Foundation (to JASJ).

PDF

122

摘要：

鼻腔内的嗅觉受体神经元具有终生再生能力；然而，头部创伤和颅底手术等一些因素会损害嗅觉神经，从而导致嗅觉功能障碍。因此，显然需要找到治疗嗅觉功能障碍的方法。生长因子被认为是一种潜在的治疗脊髓和脑损伤的有效方法。为研究血管内皮生长因子（VEGF）和血小板源性生长因子（PDGF）联合递送是否能改善小鼠嗅觉神经元的再生。实验以单侧球囊切除方法诱导小鼠嗅觉神经元退化，然后随机给予其鼻内1.5μg血管内皮生长因子（VEGF）联合血小板源性生长因子或盐水干预。生长因子治疗显著增加了损伤后第5和7天未成熟神经元的数量以及第10和14天时成熟嗅觉神经元的数量。伤后14d，与给予盐水干预的小鼠相比，生长因子联合干预的小鼠再生轴突在手术腔中延伸更大的体积。生长因子联合干预还显著减少了手术腔中的星形胶质细胞神经胶质瘢痕。结果表明，生长因子联合递送具有改善嗅觉功能障碍的潜力。

orcid:0000-0002-3702-9179(Fatemeh Chehrehasa)

关键词: 星形胶质细胞, 嗅球, 胶质瘢痕, 轴突, 生长因子, 神经元

Abstract:

The olfactory receptor neurons lining the nasal cavity have a remarkable capacity to regenerate throughout life. They are replenished continuously and their axons make new connections within the olfactory bulb.However, some factors such as head trauma and skull base surgery damage the olfactory nerve which lead to olfactory dysfunction. Losing the sense of smell has considerable effects on quality of life and life-expectancy.Therefore, there is a clear need to find a treatment for olfactory dysfunction. One such potential treatment is growth factor therapy which showed promising results in the spinal cord and brain injuries. The aim of the present study was to investigate whether combined delivery of two growth factors, vascular endothelial growth factor and platelet-derived growth factor treatment can improve the olfactory neurons regeneration in mice. The degeneration of the olfactory neurons was induced by unilateral bulbectomy. The treatment group received 1.5 μg of the combined growth factors intranasally, while the control injured group received saline. Growth factor treatment significantly increased the number of immature neurons at 5 and 7 days post injury and also the number of mature olfactory neurons at 10 and 14 days post bulbectomy.Regenerating axons extended over a larger volume in the operated cavity in the treatment group compared to control group at 14 days post bulbectomy. The growth factor treatment also significantly reduced astrocytic glia scar in the operated cavity. The results indicate that the combined delivery of the growth factors has the potential to improve olfactory dysfunction.

Key words: astrocytes, olfactory bulb, glial scar, axon, growth factors, neuron