中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2019, Vol. 14 ›› Issue (4): 683-691.doi: 10.4103/1673-5374.247472

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

牵正散促进大鼠损伤坐骨神经再生

  

  • 出版日期:2019-04-15 发布日期:2019-04-15
  • 基金资助:

    国家自然科学基金资助(31571235，31771322，81401007)；国家重点基础研究项目(973)(2014CB542201)；北京自然科学基金(7162098)；北京大学健康科学中心培养青年学者项目(BMU2017PY013)

Qian-Zheng-San promotes regeneration after sciatic nerve crush injury in rats

Zhi-Yong Wang 1 , Li-Hua Qin 1 , Wei-Guang Zhang 1 , Pei-Xun Zhang 2 , Bao-Guo Jiang 2   

  1. 1 Department of Anatomy and Histo-embryology, School of Basic Medical Sciences, Peking University, Beijing, China
    2 Department of Trauma and Orthopedics, Peking University People’s Hospital, Beijing, China
  • Online:2019-04-15 Published:2019-04-15
  • Contact: Pei-Xun Zhang, MD, PhD, zhangpeixun@126.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 31571235 (to PXZ), 31771322 (to PXZ), 81401007 (to ZYW); the National Key Basic Research Program of China (973 Program), No. 2014CB542201 (to PXZ); the Beijing Science and Technology New Star Cross Program of China, No. 2018019 (to PXZ); the Natural Science Foundation of Beijing of China, No. 7162098 (to WGZ); the Fostering Young Scholars of Peking University Health Science Center of China, No. BMU2017PY013 (to PXZ).

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摘要：

中国传统复方牵正散主要成分由白附子、僵蚕和全蝎组成，目前已被证明在帕金森病等中枢神经疾病中的治疗中有积极作用，但是否有治疗周围神经损伤的作用尚不明确。为此，实验设计以北京维通利华公司提供的2月龄雄性SD大鼠为对象，使用无齿血管钳钳夹右侧坐骨神经制作坐骨神经挤压伤模型，术后治疗组每天给予大鼠1.75 g/mL的牵正散2 mL(北京同仁堂公司)灌胃治疗1，2，4或8周，以不给药治疗的模型组作对照。以足迹分析检测大鼠肢体的坐骨神经运动功能，以电生理检测坐骨神经传导功能，以免疫组织荧光染色进行轴突计数和形态学分析、进行坐骨神经损伤远侧髓鞘再生情况及脊髓前角运动神经元数量分析。结果发现：(1)术后2，4周，与模型组相比，治疗组坐骨神经功能指数、神经传导速度、坐骨神经损伤远侧再生轴突的数量和轴突直径均升高；(2)术后2周，与模型组相比，治疗组神经纤维直径、髓鞘厚度及腰段脊髓前角运动神经元数量均升高。上述结果提示，中药复方牵正散对周围神经挤压伤后的再生有积极促进作用。

orcid: 0000-0002-8379-1607(Pei-Xun Zhang)

关键词: 中国传统中药, 挤压伤, 周围神经再生, 神经传导速度, 坐骨神经功能指数, 神经损伤, 神经修复, 方剂, 全蝎, 神经再生

Abstract:

Qian-Zheng-San, a traditional Chinese prescription consisting of Typhonii Rhizoma, Bombyx Batryticatus, Scorpio, has been found to play an active therapeutic role in central nervous system diseases. However, it is unclear whether Qian-Zheng-San has therapeutic value for peripheral nerve injury. Therefore, we used Sprague-Dawley rats to investigate this. A sciatic nerve crush injury model was induced by clamping the right sciatic nerve. Subsequently, rats in the treatment group were administered 2 mL Qian-Zheng-San (1.75 g/mL) daily as systemic therapy for 1, 2, 4, or 8 weeks. Rats in the control group were not administered Qian-Zheng-San. Rats in sham group did not undergo surgery and systemic therapy. Footprint analysis was used to assess nerve motor function. Electrophysiological experiments were used to detect nerve conduction function. Immunofluorescence staining was used to assess axon counts and morphological analysis. Im¬munohistochemical staining was used to observe myelin regeneration of the sciatic nerve and the number of motoneurons in the anterior horn of the spinal cord. At 2 and 4 weeks postoperatively, the sciatic nerve function index, nerve conduction velocity, the number of dis¬tant regenerated axons and the axon diameter of the sciatic nerve increased in the Qian-Zheng-San treatment group compared with the control group. At 2 weeks postoperatively, nerve fiber diameter, myelin thickness, and the number of motor neurons in the lumbar spinal cord anterior horn increased in the Qian-Zheng-San treatment group compared with the control group. These results indicate that Qian- Zheng-San has a positive effect on peripheral nerve regeneration.

Key words: nerve regeneration, traditional Chinese medicine, crush injury, peripheral nerve regeneration, nerve conduction velocity, sciatic function index, nerve injury, nerve repair, formula, scorpion, neural regeneration