中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2019, Vol. 14 ›› Issue (5): 896-902.doi: 10.4103/1673-5374.249238

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

血清和脑脊液tau蛋白水平可评估急性脊髓损伤的严重程度和运动功能预后

  

  • 出版日期:2019-05-15 发布日期:2019-05-15
  • 基金资助:

    国家自然科学基金(81671211，81672251)

Serum and cerebrospinal fluid tau protein level as biomarkers for evaluating acute spinal cord injury severity and motor function outcome

Ying Tang 1 , Hong-Liang Liu 2 , Ling-Xia Min 2 , Hao-Shi Yuan 3 , Lei Guo 4 , Peng-Bo Han 3 , Yu-Xin Lu 3 , Jian-Feng Zhong 3 , Dong-Lin Wang 1   

  1. 1 Key Laboratory of Biorheological Science and Technology, Ministry of Education (Chongqing University), Chongqing University Cancer Hospital, Chongqing, China
    2 Department of Rehabilitation, Southwest Hospital, Third Military Medical University, Chongqing, China
    3 Department of Spine Surgery, Second Affiliated Hospital of Xi’an Medical University, Xi’an, Shanxi Province, China
    4 Department of Orthopedics, Chinese PLA Beijing Army General Hospital, Beijing, China
  • Online:2019-05-15 Published:2019-05-15
  • Contact: Dong-Lin Wang, MD, PhD, xiaolianbei@sina.com; Jian-Feng Zhong, MD, jianfeng206@qq.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81671211, 81672251 (both to HLL).

PDF

211

摘要：

Tau蛋白作为微管相关蛋白，在神经元和轴突中有着高特异性表达，而创伤性脊髓损伤主要是神经元和轴突的损伤。为此，实验推测tau蛋白可能是一个很有前景的生物学标志物来反映脊髓损伤程度和运动功能预后。实验将160只SD雌性大鼠随机分为假手术组，轻、中、重度脊髓损伤组。后3组通过外科手术暴露T8脊髓，采用NYU-MASCIS脊椎打击器制作脊髓挫伤模型，轻、中、重度损伤组的打击高度分别为12.5，25，50 mm，假手术组仅接受椎板切除而不损伤脊髓。各组分别在术后1，6，12，24 h，3，7，14，28 d利用ELISA方法来测定血清和脑脊液tau蛋白水平，大鼠损伤程度和预后评价采用BBB评分的方法。结果发现：(1)脊髓损伤各组大鼠tau蛋白水平均在脊髓损伤后快速增加，并在损伤后12 h达到峰值；(2)相关性分析见脊髓损伤各组Tau蛋白水平和脊髓损伤程度呈正相关，且和BBB评分同样呈正相关；(3)脊髓损伤各组大鼠损伤后12 h tau蛋白水平和损伤后28 d的BBB评分呈负相关(血清：r =-0.94；脑脊液：r =-0.95)；(4)以上结果说明血清和脑脊液tau蛋白水平不仅可以反映脊髓损伤的程度，也可能与脊髓损伤的运动功能预后存在一定关系。

orcid: 0000-0003-1359-6440 (Dong-Lin Wang)
           0000-0002-8788-5942 (Jian-Feng Zhong)

关键词: 脊髓损伤, tau蛋白, 损伤严重程度, 预后, 脑脊液, 血清, 生物标志物, BBB评分, 神经再生

Abstract:

Tau protein, a microtubule-associated protein, has a high specific expression in neurons and axons. Because traumatic spinal cord injury mainly affects neurons and axons, we speculated that tau protein may be a promising biomarker to reflect the degree of spinal cord injury and prognosis of motor function. In this study, 160 female Sprague-Dawley rats were randomly divided into a sham group, and mild, moderate, and severe spinal cord injury groups. A laminectomy was performed at the T8 level to expose the spinal cord in all groups. A contusion lesion was made with the NYU-MASCIS impactor by dropping a 10 g rod from heights of 12.5 mm (mild), 25 mm (moderate) and 50 mm (severe) upon the exposed dorsal surface of the spinal cord. Tau protein levels were measured in serum and cerebrospinal fluid samples at 1, 6, 12, 24 hours, 3, 7, 14 and 28 days after operation. Locomotor function of all rats was assessed using the Basso, Beattie and Bresnahan locomotor rating scale. Tau protein concentration in the three spinal cord injury groups (both in serum and cerebrospinal fluid) rapidly increased and peaked at 12 hours after spinal cord injury. Statistically significant positive linear correlations were found between tau protein level and spinal cord injury severity in the three spinal cord injury groups, and between the tau protein level and Basso, Beattie, and Bresnahan locomotor rating scale scores. The tau protein level at 12 hours in the three spinal cord injury groups was negatively correlated with Basso, Beattie, and Bresnahan locomotor rating scale scores at 28 days (serum: r = -0.94; cerebrospinal fluid: r = -0.95). Our data suggest that tau protein levels in serum and cerebrospinal fluid might be a promising biomarker for predicting the severity and functional outcome of traumatic spinal cord injury.

Key words:  nerve regeneration, spinal cord injury, tau, injury severity, outcome, cerebrospinal fluid, serum, biomarker, Basso, Beattie, and Bresnahan locomotor rating scale, neural regeneration