中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2019, Vol. 14 ›› Issue (6): 984-990.doi: 10.4103/1673-5374.250577

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

创伤性脑损伤患者外周血内皮祖细胞数量的减少与高血糖水平有关

  

  • 出版日期:2019-06-15 发布日期:2019-06-15
  • 基金资助:

    国家自然科学基金(30772229，81200907)；天津市自然科学基金(12JCQNJC 06800)；天津中医药重点地区科技项目(2018001)；天津教委科研项目(2018ZD03)

Decreased numbers of circulating endothelial progenitor cells are associated with hyperglycemia in patients with traumatic brain injury

Hui-Jie Wei 1 , Li Liu 1 , Fang-Lian Chen 1 , Dong Wang 1 , Liang Wang 1, 2 , Zeng-Guang Wang 1 , Rong-Cai Jiang 1 , Jing-Fei Dong 1, 3 , Jie-Li Chen 1, 4 , Jian-Ning Zhang 1   

  1. 1 Department of Neurosurgery, Tianjin Medical University General Hospital; Tianjin Neurological Institute; Key Laboratory of Post-trauma Neuro-repair and Regeneration in Central Nervous System, Ministry of Education; Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin, China
    2 Department of Neurosurgery, Peking University International Hospital, Beijing, China
    3 Thrombosis Research Section, Department of Medicine, Baylor College of Medicine, Houston, TX, USA
    4 Department of Neurology, Henry Ford Health System, Detroit, MI, USA
  • Online:2019-06-15 Published:2019-06-15
  • Contact: Jian-Ning Zhang, MD, PhD, jianningzhang@hotmail.com; Jie-Li Chen, MD, jieli@neuro.hfh.edu.
  • Supported by:

    This work was supported by the National Natural Science Foundation of China, No. 30772229 (to JNZ), No. 81200907 (to HJW); the Natural Science Foundation of Tianjin of China, No. 12JCQNJC06800 (to HJW); the Science and Technology Projects in Key Areas of Traditional Chinese Medicine of Tianjin of China, No. 2018001 (to ZGW); and the Scientific Research Program Project of Tianjin Education Commission of China, No. 2018ZD03 (to ZGW).

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摘要：

有研究表明，高血糖可以下调循环血内皮祖细胞，加速内皮祖细胞的衰老以及损害内皮祖细胞的功能，但创伤性脑损伤患者血糖水平与外周血中内皮祖细胞的关系尚不明确。为此，试验采用回顾性病例分析方法，纳入2005年4月至2007年3月天津医科大学总医院神经外科和天津市环湖医院神经外科的创伤性脑损伤患者101例（18-85岁，男75例，女26例），采用流式细胞仪和全自动生化分析仪测定创伤性脑损伤患者伤后1，4，7，14和21 d的外周血内皮祖细胞数量和血糖水平，并以同期在天津医科大学总医院体检中心进行体检的健康志愿者37例（24-72岁，男28例，女9例）的外周血皮祖细胞数量和血糖水平结果作对照。与对照组相比：(1)创伤性脑损伤患者外周血内皮祖细胞的数量：在伤后第1天开始呈下降趋势，伤后第4天开始升高，伤后第7天达最高峰；(2)血糖水平与对照组相比，在伤后第1天最高，随后呈下降趋势，在伤后第7天趋于平稳。伤后第1，4，7天外周血内皮祖细胞的数量与血糖水平呈负相关(r =-0.1472，P < 0.05)；(3)结果证实：创伤性脑损伤后的患者高血糖水平与外周血内皮祖细胞数量的减少有关。研究于2005年1月经天津医科大学总医院伦理委员会批准用于生物医学研究(伦理批号：200501)及患者和志愿者的知情同意。

orcid: 0000-0002-7290-0994 (Jian-Ning Zhang)
           0000-0001-5446-9101 (Jie-Li Chen)

关键词: 内皮祖细胞, 血管修复, 血管重塑, 血管生成, 新生血管, 血糖, 高血糖, 创伤性脑损伤, 动员, 抑制, 衰老, 替代疗法, 脑损伤, 神经再生

Abstract:

Hyperglycemia reduces the number of circulating endothelial progenitor cells, accelerates their senescence and impairs their function. However, the relationship between blood glucose levels and endothelial progenitor cells in peripheral blood of patients with traumatic brain injury is unclear. In this study, 101 traumatic brain injury patients admitted to the Department of Neurosurgery, Tianjin Medical University General Hospital or the Department of Neurosurgery, Tianjin Huanhu Hospital, China, were enrolled from April 2005 to March 2007. The number of circulating endothelial progenitor cells and blood glucose levels were measured at 1, 4, 7, 14 and 21 days after traumatic brain injury by flow cytometry and automatic biochemical analysis, respectively. The number of circulating endothelial pro¬genitor cells and blood sugar levels in 37 healthy control subjects were also examined. Compared with controls, the number of circulating endothelial progenitor cells in traumatic brain injury patients was decreased at 1 day after injury, and then increased at 4 days after injury, and reached a peak at 7 days after injury. Compared with controls, blood glucose levels in traumatic brain injury patients peaked at 1 day and then decreased until 7 days and then remained stable. At 1, 4, and 7 days after injury, the number of circulating endothelial progen¬itor cells was negatively correlated with blood sugar levels (r =−0.147, P < 0.05). Our results verify that hyperglycemia in patients with traumatic brain injury is associated with decreased numbers of circulating endothelial progenitor cells. This study was approved by Ethical Committee of Tianjin Medical University General Hospital, China (approval No. 200501) on January, 2015. All subjects or guardians pro¬vided informed written consent before enrollment.

Key words: nerve regeneration, endothelial progenitor cells, vascular repair, vascular remodeling, angiogenesis, neovascularization, blood glucose, hyperglycemia, traumatic brain injury, mobilization, suppression, senescence, alternative therapy, brain damage, neural regeneration