中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2019, Vol. 14 ›› Issue (10): 1833-1840.doi: 10.4103/1673-5374.257539

• 原著:周围神经损伤修复保护与再生 • 上一篇    

坐骨神经损伤改变了脊髓前角神经元和神经胶质细胞的空间排列

  

  • 出版日期:2019-10-15 发布日期:2019-10-15
  • 基金资助:

    这项工作得到了伊朗德黑兰Shahid Beheshti医学科学大学研究基金的支持（No. 1394-373）

Sciatic nerve injury alters the spatial arrangement of neurons and glial cells in the anterior horn of the spinal cord

Ali Rashidiani-Rashidabadi 1, Mohammad Hassan Heidari 1, Ensieh Sajadi 1, Fatemeh Hejazi 2, Fatemeh Fadaei Fathabady 1,Yousef Sadeghi 1, Abbas Aliaghaei 1, Amir Raoofi 1, Mohammad-Amin Abdollahifar 1, Reza Mastery Farahni 1   

  1. 1 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
    2 Department of Polymer Engineering and Color Technology, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran
     
  • Online:2019-10-15 Published:2019-10-15
  • Contact: Reza Mastery Farahani, PhD, realmastery@hotmail.com or dr.farahani@sbmu.ac.ir; Mohammad-Amin Abdollahifar, PhD, m_amin58@yahoo.com or abdollahima@sbmu.ac.ir.
  • Supported by:

    The work was supported by the Research Vice-chancellor of Shahid Beheshti University of Medical Sciences, Tehran, Iran (No. 1394-373; to RMF).

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摘要：

神经细胞的动态变化对于维持神经组织的正常结构和功能非常重要。为了解大鼠脊髓前角细胞的三维空间分布在坐骨神经损伤后的变化，实验将大鼠分为对照组和坐骨神经横断组，每组8只。术后12周，对脊髓前角进行一阶和二阶立体视觉研究。使用叠加在脊髓切片上的偶极探针，以二阶体视学技术评估配对相关和互相关函数。结果显示，（1）与对照组相比，坐骨神经横断大鼠的脊髓前角神经胶质细胞数分别减少了7％和36％，运动神经元的平均体积和总数减少了7％和36％；但脊髓前角体积保持不变。（2）配对相关曲线和互相关曲线显示坐骨神经横断大鼠脊髓前角运动神经元与神经胶质细胞的分布呈负相关。这些发现表明，坐骨神经损伤导致脊髓前角运动神经元和神经胶质细胞的空间排列的改变，最终影响神经系统的正常功能。

orcid: 0000-0002-3037-4236 (Reza Mastery Farahani)
          0000-0001-6947-3285 (Mohammad-Amin Abdollahifar)

关键词: 坐骨神经损伤, 空间排列, 脊髓, 运动神经元, 神经胶质细胞, 轴突横切, 脊髓前角

Abstract:

The spatial arrangement of the cell is important and considered as underlying mechanism for mathematical modeling of cell to cell interaction. The ability of cells to take on the characteristics of other cells in an organism, it is important to understand the dynamical behavior of the cells. This method implements experimental parameters of the cell-cell interaction into the mathematical simulation of cell arrangement. The purpose of this research was to explore the three-dimensional spatial distribution of anterior horn cells in the rat spinal cord to examine differences after sciatic nerve injury. Sixteen Sprague-Dawley male rats were assigned to control and axotomy groups. Twelve weeks after surgery, the anterior horn was removed for first- and second-order stereological studies. Second-order stereological techniques were applied to estimate the pair correlation and cross-correlation functions using a dipole probe superimposed onto the spinal cord sections. The findings revealed 7% and 36% reductions in the mean volume and total number of motoneurons, respectively, and a 25% increase in the neuroglial cell number in the axotomized rats compared to the control rats. In contrast, the anterior horn volume remained unchanged. The results also indicated a broader gap in the pair correlation curve for the motoneurons and neuroglial cells in the axotomized rats compared to the control rats. This finding shows a negative correlation for the distribution of motoneurons and neuroglial cells in the axotomized rats. The cross-correlation curve shows a negative correlation between the motoneurons and neuroglial cells in the axotomized rats. These findings suggest that cellular structural and functional changes after sciatic nerve injury lead to the alterations in the spatial arrangement of motoneurons and neuroglial cells, finally affecting the normal function of the central nervous system. The experimental protocol was reviewed and approved by the Animal Ethics Committee of Shahid Beheshti University of Medical Sciences (approval No. IR.SBMU.MSP.REC1395.375) on October 17, 2016. 

Key words: sciatic nerve injury, spatial arrangement, spinal cord, motorneuron, neuroglial cells, axotomy, anterior horn